Techniques and Uses in Single-Cell Biology
Table of Contents Preface....................................................................................................................................................................VII Chapter 1 Single Cell Analysis of Neutrophils NETs by Microscopic LSPR Imaging System..................................1 Riyaz Ahmad Mohamed Ali, Daiki Mita, Wilfred Espulgar, Masato Saito, Masayuki Nishide, Hyota Takamatsu, Hiroyuki Yoshikawa and Eiichi Tamiya Chapter 2 Single Cell Mass Cytometry of Non-Small Cell Lung Cancer Cells Reveals Complexity of In Vivo and Three-Dimensional Models over the Petri-Dish..................................................................................................................................16 Róbert Alföldi, József Á. Balog, Nóra Faragó, Miklós Halmai, Edit Kotogány, Patrícia Neuperger, Lajos I. Nagy, Liliána Z. Fehér, Gábor J. Szebeni and László G. Puskás Chapter 3 Genomic Analysis of Localized High-Risk Prostate Cancer Circulating Tumor Cells at the Single-Cell Level................................................................................................................35 Aline Rangel-Pozzo, Songyan Liu, Gabriel Wajnberg, Xuemei Wang, Rodney J. Ouellette, Geoffrey G. Hicks, Darrel Drachenberg and Sabine Mai Chapter 4 Advances of Single-Cell Protein Analysis.......................................................................................................54 Lixing Liu, Deyong Chen, Junbo Wang and Jian Chen Chapter 5 Single-Cell RNA Sequencing and its Combination with Protein and DNA Analyses........................................................................................................................................................74 Jane Ru Choi, Kar Wey Yong, Jean Yu Choi and Alistair C. Cowie Chapter 6 A Single-Neuron: Current Trends and Future Prospects............................................................................108 Pallavi Gupta, Nandhini Balasubramaniam, Hwan-You Chang, Fan-Gang Tseng and Tuhin Subhra Santra Chapter 7 Highly Sensitive and Multiplexed In-Situ Protein Profiling with Cleavable Fluorescent Streptavidin.................................................................................................................153 Renjie Liao, Thai Pham, Diego Mastroeni, Paul D. Coleman, Joshua Labaer and Jia Guo Chapter 8 Single-Nucleus Sequencing of an Entire Mammalian Heart: Cell Type Composition and Velocity.................................................................................................................................167 Markus Wolfien, Anne-Marie Galow, Paula Müller, Madeleine Bartsch, Ronald M. Brunner, Tom Goldammer, Olaf Wolkenhauer, Andreas Hoeflich and Robert David Chapter 9 The Role of Single-Cell Technology in the Study and Control of Infectious Diseases.............................................................................................................................................173 Weikang Nicholas Lin, Matthew Zirui Tay, Ri Lu, Yi Liu, Chia-Hung Chen and Lih Feng Cheow Chapter 10 Single-Cell Transcriptomes Reveal Characteristic Features of Mouse Hepatocytes with Liver Cholestatic Injury.......................................................................................201 Na Chang, Lei Tian, Xiaofang Ji, Xuan Zhou, Lei Hou, Xinhao Zhao, Yuanru Yang, Lin Yang and Liying Li Chapter 11 Total mRNA Quantification in Single Cells: Sarcoma Cell Heterogeneity.............................................218 Emma Jonasson, Lisa Andersson, Soheila Dolatabadi, Salim Ghannoum, Pierre Åman and Anders Ståhlberg Chapter 12 A Microfluidic Single-Cell Cloning (SCC) Device for the Generation of Monoclonal Cells.................................................................................................................................................229 Chuan-Feng Yeh, Ching-Hui Lin, Hao-Chen Chang, Chia-Yu Tang, Pei-Tzu Lai and Chia-Hsien Hsu Permissions List of Contributors Index Contents