ADIPOSE TISSUE SAMPLING METHODS
CONTENTS 1 CHOOSING AN ADIPOSE TISSUE DEPOT FOR SAMPLING 2 MORPHOLOGIC TECHNIQUES FOR THE STUDY OF BROWN ADIPOSE TISSUE AND WHITE ADIPOSE TISSUE 3 CONFOCAL MICROSCOPY OF ADIPOCYTES 4 CELLULARITY MEASUREMENTS 5 SUBCELLULAR FRACTIONATION OF ADIPOCYTES AND 3T3-L1 CELLS 6 QUANTIFICATION OF LIPID-RELATED MRNAS BY REVERSE TRANSCRIPTION-COMPETITIVE POLYMERASE CHAIN REACTION IN HUMAN WHITE ADIPOSE TISSUE BIOPSIES 7 GLYCOGEN SYNTHASE ACTIVITY IN ADIPOSE TISSUE 8 ASSAYS OF LIPOLYTIC ENZYMES 9 ASSAYS OF LIPOGENIC ENZYMES 10 ASSAYS OF ADRENERGIC RECEPTORS 11 TRANSFECTION OF ADIPOCYTES AND PREPARATION OF NUCLEAR EXTRACTS 12 ASSAY OF MEMBRANE TRANSPORT OF LONG-CHAIN FATTY ACIDS BY ADIPOCYTES 13 ASSAYS OF GLUCOSE ENTRY, GLUCOSE TRANSPORTER AMOUNT, AND TRANSLOCATION 14 METHODS TO STUDY PHOSPHORYLATION AND ACTIVATION OF THE HORMONE-SENSITIVE ADIPOCYTE PHOSPHODIESTERASE TYPE 3B IN RAT ADIPOCYTES 15 MEASUREMENTS OF GLUCOSE CONVERSION TO ITS METABOLITES 16 METABOLITE AND ION FLUXES AND ION CHANNELS IN BROWN AND WHITE ADIPOCYTES 17 CULTURE OF ADIPOSE TISSUE AND ISOLATED ADIPOCYTES 18 CULTURES OF ADIPOSE PRECURSOR CELLS FROM BROWN ADIPOSE TISSUE AND OF CLONAL BROWN-ADIPOCYTE-LIKE CELL LINES 19 CULTURES OF ADIPOSE PRECURSOR CELLS AND CELLS OF CLONAL LINES FROM ANIMAL WHITE ADIPOSE TISSUE 20 CULTURES OF HUMAN ADIPOSE PRECURSOR CELLS 21 MEASUREMENTS OF PEPTIDE AND NONPEPTIDE SECRETORY PRODUCTS FROM ADIPOCYTES 22 ASSESSMENT OF WHITE ADIPOSE TISSUE METABOLISM BY MEASUREMENT OF ARTERIOVENOUS DIFFERENCES 23 MEASUREMENT OF ADIPOSE TISSUE BLOOD FLOW 24 RESPIRATORY AND THERMOGENIC CAPACITIES OF CELLS AND MITOCHONDRIA FROM BROWN AND WHITE ADIPOSE TISSUE 25 MEASUREMENTS OF WHITE ADIPOSE TISSUE METABOLISM BY MICRODIALYSIS TECHNIQUE 26 IMMUNOASSAY MEASUREMENTS OF LEPTIN