FUNDAMENTALS OF CAPILLARY ELECTROPHORESIS THEORY

By G. MAMIS

CONTENTS FUNDAMENTALS OF ELECTROPHORESIS OVERVIEW ON ELECTROPHORESIS SEPARATION MEDIA ELECTROPHORESIS RESOLUTION AND SHARPNESS DETECTION OF RESOLVED BANDS COMPARISON BETWEEN ELECTROPHORESIS AND OTHER SEPARATION METHODS ULTRACENTRIFUGATION AND CHROMATOGRAPHY IONS AND POLYIONS ELECTROLYTES ELECTRIC DOUBLE LAYER OF A CHARGED PARTICLE TWO RADII AND TWO ELECTRIC POTENTIALS OF A CHARGED PARTICLE PROTEINS AND NUCLEIC ACIDS FORM POLYIONS IN A SOLUTION STRUCTURE AND CONFORMATION OF PROTEINS STRUCTURE AND CONFORMATION OF NUCLEIC ACIDS ELECTROPHORESIS IS RUNNING IN BUFFERS BUFFERS BUFFERS USED IN ELECTROPHORESIS BIOLOGICAL BUFFERS THE POLYIONS MOVE IN ELECTRIC FIELD IONIC AND POLYIONIC MOBILITY EFFECTIVE MOBILITY EQUATIONS OF POLYIONIC MOBILITY MOBILITIES OF IONS USED IN ELECTROPHORETIC METHODS ELECTROPHORESIS IS CARRIED OUT IN DIFFERENT SOLID MEDIA CELLULOSE ACETATE STARCH GEL AGAROSE GEL POLYACRYLAMIDE GEL GENERAL THEORY OF ELECTROPHORESIS WHAT IS THE POLYIONIC MOBILITY DEPENDING ON? WHAT IS THE ELECTRIC FIELD STRENGTH DEPENDING ON? IONIC BOUNDARIES REGULATING FUNCTION DIFFUSION JOULE HEATING ELECTROPHORESIS INSTRUMENTATION ELECTROPHORESIS CELLS POWER SUPPLIES THERMOSTATS DENSITOMETERS GEL CASTING SYSTEMS BUFFER MIXERS COOLING DEVICES BLOTTERS EQUIPMENT FOR SEMIAUTOMATIC ELECTROPHORESIS EQUIPMENT FOR PREPARATIVE ELECTROPHORESIS CLASSIFICATION OF ELECTROPHORETIC METHODS ZONE ELECTROPHORESIS ISOTACHOPHORESIS ISOELECTRIC FOCUSING DIELECTROPHORESIS PROBLEMS 1 FUNDAMENTALS OF ELECTROPHORESIS ELECTROPHORESIS OF PROTEINS CELLULOSE ACETATE ELECTROPHORESIS OF PROTEINS THEORY OF CELLULOSE ACETATE ELECTROPHORESIS OF PROTEINS PRACTICE OF CELLULOSE ACETATE ELECTROPHORESIS OF PROTEINS PROTOCOLS TROUBLESHOOTING AGAROSE GEL ELECTROPHORESIS OF PROTEINS THEORY OF AGAROSE GEL ELECTROPHORESIS OF PROTEINS AGAROSE GEL ELECTROPHORESIS OF SERUM PROTEINS AGAROSE GEL ELECTROPHORESIS OF LIPOPROTEINS AGAROSE GEL ELECTROPHORESIS OF HEMOGLOBINS AGAROSE GEL ELECTROPHORESIS OF CEREBROSPINAL FLUID PROTEINS ELECTROPHORESIS OF CREATINE KINASE ELECTROPHORESIS OF LACTATE DEHYDROGENASE AGAROSE GEL ELECTROPHORESIS OF URINARY PROTEINS SEMI-DENATURING DETERGENT AGAROSE GEL ELECTROPHORESIS IMMUNOELECTROPHORESIS IMMUNOGLOBULINS IMMUNODIFFUSION ELECTROPHORESIS ACCORDING TO GRABAR AND WILLIAMS ROCKET IMMUNOELECTROPHORESIS ACCORDING TO LAURELL COUNTER-CURRENT IMMUNOELECTROPHORESIS ACCORDING TO ESTELA AND HEINRICHS CROSSED IMMUNOELECTROPHORESIS ACCORDING TO CLARKE AND FREEMAN IMMUNOFIXATION IMMUNOPRINTING AFFINITY ELECTROPHORESIS THEORY OF AFFINITY ELECTROPHORESIS LECTIN AFFINITY ELECTROPHORESIS SACCHARIDE AFFINITY ELECTROPHORESIS AFFINITY SUPPORTED MOLECULAR MATRIX ELECTROPHORESIS PHOSPHATE AFFINITY ELECTROPHORESIS CAPILLARY AFFINITY ELECTROPHORESIS AFFINITY-TRAP ELECTROPHORESIS CHARGE SHIFT ELECTROPHORESIS MOBILITY SHIFT ELECTROPHORESIS POLYACRYLAMIDE GEL ZONE ELECTROPHORESIS OF PROTEINS THEORY OF POLYACRYLAMIDE GEL ZONE ELECTROPHORESIS OF PROTEINS HOMOGENEOUS ZONE ELECTROPHORESIS GRADIENT POLYACRYLAMIDE GEL ELECTROPHORESIS OF PROTEINS THEORY OF THE GRADIENT GEL ZONE ELECTROPHORESIS FERGUSON PLOTS DETERMINATION OF STOKES RADII AND MASSES OF NATIVE PROTEINS GRADIENT POLYACRYLAMIDE GEL ZONE ELECTROPHORESIS OF MASS AND CHARGE ISOMERS OF PROTEINS BLUE NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS ISOTACHOPHORESIS OF PROTEINS THEORY OF ISOTACHOPHORESIS OF PROTEINS DISC-ELECTROPHORESIS OF PROTEINS THEORY OF DISC-ELECTROPHORESIS DISC-ELECTROPHORESIS ACCORDING TO ORNSTEIN AND DAVIS DISC-ELECTROPHORESIS ACCORDING TO ALLEN ET AL DISC-ELECTROPHORESIS IN ONE BUFFER AT TWO PH VALUES SDS ELECTROPHORESIS OF PROTEINS THEORY OF DETERGENT ELECTROPHORESIS OF PROTEINS SAMPLE PREPARATION FOR SDS POLYACRYLAMIDE GEL ELECTROPHORESIS SDS POLYACRYLAMIDE GEL ELECTROPHORESIS DETERMINATION OF MOLECULAR MASSES OF SDS-DENATURED PROTEINS FAST PARALLEL PROTEOLYSIS FREE-FLOW ELECTROPHORESIS OF PROTEINS GENERAL THEORY OF FREE-FLOW ELECTROPHORESIS TYPES OF FREE-FLOW ELECTROPHORESIS DEVICE TECHNOLOGY OF FREE-FLOW ELECTROPHORESIS DETECTION SYSTEM OF FREE-FLOW ELECTROPHORESIS APPLICATIONS OF FREE-FLOW ELECTROPHORESIS ISOELECTRIC FOCUSING OF PROTEINS THEORY OF ISOELECTRIC FOCUSING ISOELECTRIC FOCUSING WITH CARRIER AMPHOLYTES ISOELECTRIC FOCUSING IN IMMOBILIZED PH GRADIENTS ISOELECTRIC FOCUSING BY LIVING CELLS ELECTRIC FIELD GRADIENT FOCUSING APPLICATIONS OF ISOELECTRIC FOCUSING CAPILLARY ELECTROPHORESIS OF PROTEINS THEORY OF CAPILLARY ELECTROPHORESIS OF PROTEINS INSTRUMENTATION PRACTICE OF CAPILLARY ELECTROPHORESIS TYPES OF CAPILLARY ELECTROPHORESIS APPLICATIONS OF CAPILLARY ELECTROPHORESIS TWO-DIMENSIONAL ELECTROPHORESIS THEORY OF TWO-DIMENSIONAL ELECTROPHORESIS ISOELECTRIC FOCUSING IN THE FIRST DIMENSION SDS DISC-ELECTROPHORESIS IN THE SECOND DIMENSION DETECTION AND EVALUATION OF PROTEINS IN 2D PHEROGRAMS PREPARATIVE ELECTROPHORESIS OF PROTEINS PREPARATIVE DISC-ELECTROPHORESIS PREPARATIVE ISOELECTRIC FOCUSING QPNC-PAGE MICROCHIP ELECTROPHORESIS OF PROTEINS ADVANTAGES AND DISADVANTAGES OF MICROCHIP ELECTROPHORESIS MICROCHIP MATERIALS MICROCHIP FABRICATION ZONE ELECTROPHORESIS ON MICROCHIP ISOTACHOPHORESIS ON MICROCHIP ISOELECTRIC FOCUSING ON MICROCHIP 2D ELECTROPHORESIS ON MICROCHIP PROTEIN SEPARATION ON MICROCHIP MICROCHIPS IN CLINICAL DIAGNOSTICS BLOTTING OF PROTEINS THEORY OF PROTEIN BLOTTING BLOT MEMBRANES TRANSFER OF PROTEINS BLOCKING DETECTION MAKING BLOT MEMBRANES TRANSPARENT BLOTTING TECHNIQUES BLOTTING OF HIGH-MOLECULAR-MASS PROTEINS FROM AGAROSE GELS THEORY OF DIELECTROPHORESIS DEP TECHNOLOGY APPLICATIONS OF DIELECTROPHORESIS DIELECTROPHORESIS FIELD-FLOW FRACTIONATION QUALITATIVE EVALUATION OF PROTEIN PHEROGRAMS FIXING OF PROTEINS STAINING OF PROTEINS AUTORADIOGRAPHY OF PROTEINS FLUOROGRAPHY OF PROTEINS DOUBLE ISOTOPE DETECTION PHOSPHOR IMAGING DESTAINING OF GEL BACKGROUND DRYING OF GELS DOCUMENTATION OF PROTEIN PHEROGRAMS QUANTITATIVE EVALUATION OF A PROTEIN PHEROGRAM DENSITOMETRY SCANNING PRECAST GELS FOR PROTEIN ELECTROPHORESIS REHYDRATABLE GELS PRECAST AGAROSE GELS PRECAST POLYACRYLAMIDE GELS PROBLEMS 2 ELECTROPHORESIS OF PROTEINS ELECTROPHORESIS OF NUCLEIC ACIDS BUFFERS FOR ELECTROPHORESIS OF NUCLEIC ACIDS POLYMERASE-CHAIN REACTION PRINCIPLE COMPONENTS PROCEDURE STAGES ADVANTAGES LIMITATIONS APPLICATIONS AGAROSE GEL ELECTROPHORESIS OF NUCLEIC ACIDS THEORY OF AGAROSE GEL ELECTROPHORESIS OF NUCLEIC ACIDS NATIVE AGAROSE GEL ELECTROPHORESIS OF NUCLEIC ACIDS DENATURING AGAROSE GEL ELECTROPHORESIS OF NUCLEIC ACIDS PULSED-FIELD GEL ELECTROPHORESIS OF NUCLEIC ACIDS ELUTION OF DNA FROM AGAROSE GELS VISUALIZATION OF NUCLEIC ACIDS NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS OF NUCLEIC ACIDS ZONE POLYACRYLAMIDE GEL ELECTROPHORESIS OF NATIVE NUCLEIC ACIDS DISC-ELECTROPHORESIS OF NATIVE NUCLEIC ACIDS DISC-ELECTROPHORESIS OF DOUBLE-STRANDED PCR PRODUCTS ELECTROPHORETIC MOBILITY SHIFT ASSAY CLINICAL APPLICATION DENATURING POLYACRYLAMIDE GEL DISC-ELECTROPHORESIS OF NUCLEIC ACIDS THEORY OF THE DENATURING POLYACRYLAMIDE GEL ELECTROPHORESIS OF NUCLEIC ACIDS DNA SEQUENCING SINGLE-STRAND CONFORMATION POLYMORPHISM DNASE FOOTPRINTING ASSAY NUCLEASE PROTECTION ASSAY RNA SEPARATION PRIMER EXTENSION ASSAY TEMPERATURE AND DENATURING GRADIENT GEL ELECTROPHORESIS OF NUCLEIC ACIDS TEMPERATURE GRADIENT GEL ELECTROPHORESIS OF NUCLEIC ACIDS DENATURING GRADIENT GEL ELECTROPHORESIS OF NUCLEIC ACIDS CAPILLARY ELECTROPHORESIS OF NUCLEIC ACIDS THEORY OF CAPILLARY ELECTROPHORESIS INSTRUMENTATION FOR CAPILLARY ELECTROPHORESIS RUNNING CAPILLARY ELECTROPHORESIS PULSED-FIELD CAPILLARY ELECTROPHORESIS MICROCHIP ELECTROPHORESIS OF NUCLEIC ACIDS THEORY OF MICROCHIP ELECTROPHORESIS OF NUCLEIC ACIDS CONSTRUCTION OF A MICROCHIP FOR ELECTROPHORESIS OF NUCLEIC ACIDS FABRICATION OF A MICROCHIP FOR DNA ELECTROPHORESIS RUNNING MICROCHIP ELECTROPHORESIS OF NUCLEIC ACIDS APPLICATIONS OF DNA MICROCHIP ELECTROPHORESIS BLOTTING OF NUCLEIC ACIDS BLOTTING PRINCIPLES SOUTHERN BLOTTING NORTHERN BLOTTING MIDDLE-EASTERN BLOTTING SURFACE ELECTROPHORESIS THEORY OF SURFACE ELECTROPHORESIS DEVICES FOR SURFACE ELECTROPHORESIS EVALUATION OF NUCLEIC ACID PHEROGRAMS COUNTERION DYE STAINING OF NUCLEIC ACIDS SILVER STAINING OF NUCLEIC ACIDS FLUORESCENCE METHODS FOR DETECTING NUCLEIC ACIDS AUTORADIOGRAPHY OF NUCLEIC ACIDS LABELING NUCLEIC ACIDS WITH PROTEINS ABSORPTION SPECTROSCOPY OF NUCLEIC ACIDS PRECAST GELS FOR NUCLEIC ACID ELECTROPHORESIS PROBLEMS 3 ELECTROPHORESIS OF NUCLEIC ACIDS IONTOPHORESIS THEORY OF IONTOPHORESIS FACTORS AFFECTING IONTOPHORESIS PHYSICOCHEMICAL FACTORS BIOLOGICAL FACTORS CALCULATING THE IONTOPHORETIC CURRENT REVERSE IONTOPHORESIS ELECTROOSMOSIS IONTOPHORESIS DEVICE BATTERY ELECTRODES RESERVOIR DIAGNOSTIC IONTOPHORESIS THERAPEUTIC IONTOPHORESIS TRANSDERMAL IONTOPHORESIS OCULAR IONTOPHORESIS DANGER DURING ELECTROPHORESIS DANGER BY OBJECTS TO BE EXAMINED DANGER BY SEPARATION MEDIUM DANGER DURING ELECTROPHORETIC TREATMENT DANGER DURING ELECTROPHORESIS RUN FUTURE OF ELECTROPHORESIS PROTEIN ELECTROPHORESIS GEL ELECTROPHORESIS OF PROTEINS PROTEIN MICROCHIP ELECTROPHORESIS NUCLEIC ACID ELECTROPHORESIS GEL ELECTROPHORESIS OF NUCLEIC ACIDS DNA MICROCHIP ELECTROPHORESIS DIGITAL DOCUMENTATION HISTORY OF ELECTROPHORESIS AND IONTOPHORESIS SI UNITS AND PHYSICAL CONSTANTS FOR ELECTROPHORESIS REAGENTS FOR ELECTROPHORESIS RECIPES FOR ELECTROPHORESIS SOLUTIONS SOLUTION OF PROBLEMS