AN INTRODUCTORY TREATISE ON THE NATURE AND PROPERTIES OF LIGHT, AND ON OPTICAL INSTRUMENTS
1 The Physical Principles of Light Propagation and Light–Matter Interactions 1 2 Design, Alignment, and Usage of Infinity-Corrected Microscope 17 3 Epifluorescence Microscopy 57 4 Basic Digital Image Acquisition, Design, Processing, Analysis, Management, and Presentation 77 5 Confocal Microscopy 105 6 Live-Cell Imaging: A Balancing Act Between Speed, Sensitivity, and Resolution 139 7 Structured Illumination Microscopy 173 8 Stimulated Emission Depletion Microscopy 195 9 Two-Photon Imaging 215 10 Modern Microscopy Image Analysis: Quantifying Colocalization on a Mobile Device 243 11 Spinning Disk Microscopy 263 12 Light-Sheet Fluorescence Microscopy 279 13 Localization Microscopy: A Review of the Progress in Methods and Applications 299 Correction to: Live-Cell Imaging: A Balancing Act Between Speed, Sensitivity, and Resolution C1