HPLC OF PEPTIDES AND PROTEINS: METHODS AND PROTOCOLS
CONTENTS PART I MODES OF HPLC HPLC OF PEPTIDES AND PROTEINS: BASIC THEORY AND METHODOLOGY 2 REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY I ON-EXCHANGE CHROMATOGRAPHY H IGH-PERFORMANCE HYDROPHOBIC INTERACTION CHROMATOGRAPHY 5 GEL FILTRATION CHROMATOGRAPHY H YDROPHILIC INTERACTION CHROMATOGRAPHY I MMOBILIZED METAL ION AFFINITY CHROMATOGRAPHY OF PROTEINS I MMUNOAFFINITY CHROMATOGRAPHY OF PROTEINS LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY AND TANDEM MASS SPECTROMETRY OF PEPTIDES AND PROTEINS PART II PREPARATIVE METHODOLOGIES 10 CAPILLARY SEPARATIONS 11 MICROPREPARATIVE HPLC OF PEPTIDES AND PROTEINS 12 MULTIDIMENSIONAL HPLC PURIFICATION OF PROTEINS ANALYTICAL HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY 14 PREP/SEMIPREP SEPARATIONS OF PEPTIDES 15 LARGE-SCALE PROTEIN CHROMATOGRAPHY PART III APPLICATIONS 16 PROTEOLYTIC PEPTIDE MAPPING 17 MASS SPECTROMETRIC CHARACTERIZATION OF POSTTRANSLATIONALLY MODIFIED PROTEINS—PHOSPHORYLATION ANALYSES OF GLYCOPEPTIDES AND GLYCOPROTEINS BY LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY AND LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY 19 HPLC IN THE ANALYSIS OF PEPTIDE METABOLISM 20 ISOLATION AND CHARACTERIZATION OF NATURALLY PROCESSED MHC-BOUND PEPTIDES FROM THE SURFACE OF ANTIGEN-PRESENTING CELLS 21 AUSTRALIAN FUNNEL-WEB SPIDER VENOM ANALYZED WITH ON-LINE RP-HPLC TECHNIQUES 22 HPLC AND MASS SPECTROMETRY OF INTRINSIC MEMBRANE PROTEINS 23 IGG PURIFICATION 24 DNA–BINDING PROTEINS: LC–MS TO IDENTIFY KEY DOMAINS IN RNA POLYMERASE–PROMOTER INTERACTIONS 25 SENSITIVE ENZYMATIC ANALYSIS OF HISTIDINE DECARBOXYLASE USING HPLC 26 AUTOMATED VS MANUAL PROFILING OF PEPTIDE LIBRARIES BY MASS SPECTROMETRY 27 PROTEOME ANALYSIS