BACTERIA FROM FISH AND OTHER AQUATIC ANIMALS: A PRACTICAL IDENTIFICATION MANUAL
CONTENTS AQUATIC ANIMAL SPECIES AND ORGANISM RELATIONSHIP 1 1 HOST SPECIES, BACTERIA AND DISEASE 1 2 BACTERIAL DISEASES 1 3 BACTERIA AND RELATIONSHIP TO HOST 1 4 TAXONOMY AND DISEASE STATUS OF BACTERIA BACTERIOLOGICAL CULTURE TECHNIQUES: MICROSCOPY, CULTURE AND IDENTIFICATION 2 1 SPECIMEN COLLECTION AND SUBMISSION 2 2 CULTURE AND INCUBATION 2 3 EXAMINATION OF CULTURE PLATES 2 4 BIOCHEMICAL IDENTIFICATION TESTS 2 5 INOCULATION OF BIOCHEMICAL IDENTIFICATION SETS 2 6 API IDENTIFICATION SYSTEMS INTERPRETATION OF BIOCHEMICAL IDENTIFICATION TESTS AND SETS 3 1 CONVENTIONAL MEDIA: ‘BIOCHEM SET’ 3 2 IDENTIFICATION TESTS AND THEIR INTERPRETATION 3 3 USING THE BIOCHEMICAL IDENTIFICATION TABLES 3 4 INTERPRETATION AND IDENTIFICATION OF GENERA AND SPECIES 3 5 ANTISERA AVAILABLE BIOCHEMICAL IDENTIFICATION TABLES 4 1 RESULTS FOR CONVENTIONAL BIOCHEMICAL TESTS – ‘BIOCHEM SET’ 4 2 RESULTS FOR API KITS TECHNICAL METHODS 5 1 TOTAL BACTERIAL COUNT (TBC) 5 2 MICROSCOPY 5 3 STORAGE OF ISOLATES TECHNIQUES FOR THE MOLECULAR IDENTIFICATION OF BACTERIA 6 1 MOLECULAR IDENTIFICATION BY PCR USING SPECIFIC PRIMERS 6 2 PCR PROTOCOLS 6 3 MOLECULAR IDENTIFICATION BY 16S RDNA SEQUENCING 6 4 FLUORESCENCE IN SITU HYBRIDIZATION (FISH) PREPARATION OF MEDIA FOR CULTURE AND IDENTIFICATION 7 1 GENERAL ISOLATION AND SELECTIVE MEDIA 7 2 BIOCHEMICAL TEST MEDIA