HIGH-RESOLUTION ELECTROPHORESIS AND IMMUNOFIXATION: TECHNIQUES AND INTERPRETATION
CONTENTS PART I ASSESSING IDPS IN THE LIVING CELL DETERMINATION OF IUP BASED ON SUSCEPTIBILITY FOR DEGRADATION BY DEFAULT IN-CELL NMR OF INTRINSICALLY DISORDERED PROTEINS IN PROKARYOTIC CELLS IN-CELL NMR IN XENOPUS LAEVIS OOCYTES IN-CELL NMR IN MAMMALIAN CELLS: PART FOURIER TRANSFORM INFRARED MICROSPECTROSCOPY OF COMPLEX BIOLOGICAL SYSTEMS: FROM INTACT CELLS TO WHOLE ORGANISMS STUDYING IDP STABILITY AND DYNAMICS BY FAST RELAXATION IMAGING IN LIVING CELLS PART II NMR-BASED TECHNIQUES MEASUREMENT AND ANALYSIS OF NMR RESIDUAL DIPOLAR COUPLINGS FOR THE STUDY OF INTRINSICALLY DISORDERED PROTEINS DISTANCE INFORMATION FOR DISORDERED PROTEINS FROM NMR AND ESR MEASUREMENTS USING PARAMAGNETIC SPIN LABELS USING CHEMICAL SHIFTS TO ASSESS TRANSIENT SECONDARY STRUCTURE AND GENERATE ENSEMBLE STRUCTURES OF INTRINSICALLY DISORDERED PROTEINS MAGIC ANGLE SPINNING SOLID-STATE NMR EXPERIMENTS FOR STRUCTURAL CHARACTERIZATION OF PROTEINS WIDE-LINE NMR AND PROTEIN HYDRATION 5-FLUORO-D,L-TRYPTOPHAN AS A DUAL NMR AND FLUORESCENT PROBE OF A-SYNUCLEIN ALPHA PROTON DETECTION BASED BACKBONE ASSIGNMENT OF INTRINSICALLY DISORDERED PROTEINS PART III VIBRATIONAL SPECTROSCOPY FOURIER TRANSFORM INFRARED SPECTROSCOPY OF INTRINSICALLY DISORDERED PROTEINS: MEASUREMENT PROCEDURES AND DATA ANALYSES MONITORING STRUCTURAL TRANSITIONS IN IDPS BY VIBRATIONAL SPECTROSCOPY OF CYANYLATED CYSTEINE STRUCTURE ANALYSIS OF UNFOLDED PEPTIDES I: VIBRATIONAL CIRCULAR DICHROISM SPECTROSCOPY STRUCTURAL ANALYSIS OF UNFOLDED PEPTIDES BY RAMAN SPECTROSCOPY ISOTOPE-EDITED INFRARED SPECTROSCOPY PART IV OTHER SPECTROSCOPIC TECHNIQUES MONITORING STRUCTURAL TRANSITIONS IN IDPS BY SITE-DIRECTED SPIN LABELING EPR SPECTROSCOPY CIRCULAR DICHROISM TECHNIQUES FOR THE ANALYSIS OF INTRINSICALLY DISORDERED PROTEINS AND DOMAINS DECONSTRUCTING TIME-RESOLVED OPTICAL ROTATORY DISPERSION KINETIC MEASUREMENTS OF CYTOCHROME C FOLDING: FROM MOLTEN GLOBULE TO THE NATIVE STATE THE USE OF UV–VIS ABSORPTION SPECTROSCOPY FOR STUDIES OF NATIVELY DISORDERED PROTEINS INTRINSIC FLUORESCENCE OF INTRINSICALLY DISORDERED PROTEINS BINDING STOICHIOMETRY AND AF?NITY OF FLUORESCENT DYES TO PROTEINS IN DIFFERENT STRUCTURAL STATES FLUORESCENCE LIFETIME MEASUREMENTS OF INTRINSICALLY UNSTRUCTURED PROTEINS: APPLICATION TO A-SYNUCLEIN ENSEMBLE FRET METHODS IN STUDIES OF INTRINSICALLY DISORDERED PROTEINS FLUORESCENCE CORRELATION SPECTROSCOPY TO DETERMINE THE DIFFUSION COEF?CIENT OF A-SYNUCLEIN AND FOLLOW EARLY OLIGOMER FORMATION