HISTOCHEMICAL TECHNIQUES

By I. HATIE

INTRODUCTION STATE OF THE ART LASER TISSUE INTERACTION THE PROCESS OF TISSUE ABLATION WITH PULSED LASERS LASERS AS SCALPELS IN VARIOUS SURGICAL SPECIALTIES CHALLENGES OF LASER SURGERY OPTICAL TECHNIQUES FOR TISSUE DISCRIMINATION TISSUE ELEMENTAL COMPOSITION HEALTHY AND CANCEROUS TISSUES TECHNIQUES FOR MONITORING THE ELEMENTAL DISTRIBUTIONS IN TISSUES LASER?INDUCED BREAKDOWN SPECTROSCOPY (LIBS) FUNDAMENTALS OF LIBS QUANTITATIVE LIBS BASED ON CALIBRATION?CURVE CALIBRATION?FREE LIBS (CF?LIBS) FOR QUANTITATIVE ANALYSIS APPLICATIONS OF LIBS OTHER STUDIES OF LIBS AS DIAGNOSIS TOOL FOR BIOLOGICAL APPLI? CATIONS CLASSI?CATION OF LIBS SPECTRA PRINCIPAL COMPONENT ANALYSIS (PCA) LINEAR DISCRIMINANT ANALYSIS (LDA) BINARY CLASSI?CATION MODEL USING RECEIVER OPERATING CHAR? ACTERISTICS (ROC) OBJECTIVE EXPERIMENTAL SETUP AND METHODS PREPARATION OF EXPERIMENTAL TISSUE SAMPLES EXPERIMENTAL SETUP CLASSI?CATION OF TISSUE LIBS SPECTRA PRE?PROCESSING OF LIBS SPECTRAL DATA MEASUREMENTS OF ELEMENTS IN EX?VIVO PORCINE?TISSUES USING LIBS SOFT TISSUES NERVE PAROTID GLAND FAT MUSCLE SKIN SOFT AND HARD BONE TISSUES CORTICAL BONE CANCELLOUS BONE CARTILAGE DENTAL TISSUES SUMMARY MEASUREMENT OF ELEMENTS IN TISSUE USING LIBS STATISTICAL CLASSIFICATION MODELS FOR SOFT AND HARD TISSUE DIFFER? ENTIATION NERVE AND GLAND NERVE AND FAT CORTICAL BONE AND CARTILAGE DENTAL, BONE AND SOFT TISSUES OF THE ORAL CAVITY TISSUE TYPE MAPPING BASED ON CLASSI?CATION LIBS BASED TISSUE DI?ERENTIATION IN COMBINATION WITH A SURGICAL ER:YAG LASER SUMMARY OF STATISTICAL CLASSI?CATION MODELS FOR SOFT AND HARD TISSUE DI?ERENTIATION TRANSFER STUDY OF EX?VIVO TISSUE DIFFERENTIATION MODEL FROM PORCINE TO MICE TISSUES COMPARISON OF THE LIBS SPECTRA OF MICE AND PORCINE TISSUES CLASSI?CATION PERFORMANCE OF THE PORCINE MODEL ON MICE TISSUE SPECTRA SUMMARY OF TRANSFERABILITY OF CLASSI?CATION MODEL FROM PORCINE TO MICE TISSUES TWO?DIMENSIONAL(2D) MAPPING OF ELEMENTS AS AN IMAGING MODALITY ELEMENTAL DISTRIBUTION AS AN IMAGING MODALITY FRESH TISSUES TUMOR WITH BULGED SURFACE TUMOR WITH ?AT SURFACE PROCESSED TISSUES PARA?N EMBEDDED MOUSE TISSUES EPOXY?RESIN EMBEDDED MOUSE TISSUES SUMMARY OF TWO?DIMENSIONAL(2D) MAPPING OF ELEMENTS AS AN IMAG? ING MODALITY VALIDATION OF LIBS SPECTRA BY THE PHYSICAL PROPERTIES OF THE PLASMA MEASUREMENT PROCEDURE EXISTENCE OF OPTICALLY THIN WINDOW DETERMINATION OF PLASMA TEMPERATURE SUMMARY OF VALIDATION OF LIBS SPECTRA BY PLASMA PROPERTIES 10 SUMMARY AND OUTLOOK 11 ZUSAMMENFASSUNG UND AUSBLICK THEMA ZUSAMMENFASSUNG