IMMUNOLOGY AND AGING
CONTENTS UNIT I MICROSCOPE AND BASIC MICROBIOLOGICAL TECHNIQUES INTRODUCTION 1 COMPOUND MICROSCOPE 2 DARKGROUND MICROSCOPY 3 MEASUREMENT OF MICROORGANISMS 4 HANGING DROP PREPARATION 5 ISOLATION OF PURE CULTURES UNIT II BACTERIAL STAINING 6 SIMPLE STAINING 7 GRAM’S STAINING 8 ACID FAST STAINING 9 ALBERT’S STAINING 10 CAPSULE STAINING 11 SPORE STAINING 12 NEGATIVE STAINING UNIT III CULTIVATION OF BACTERIA 13 MEDIA FOR ROUTINE CULTIVATION OF BACTERIA 14 TEMPERATURE REQUIREMENT FOR GROWTH OF BACTERIA 15 PH REQUIREMENT FOR GROWTH OF BACTERIA 16 OXYGEN REQUIREMENT FOR GROWTH OF BACTERIA 17 CULTURE OF ANAEROBIC BACTERIA 18 STERILIZATION OF COMMONLY USED CULTURE MEDIA 19 ANTISEPTICS AND DISINFECTANTS UNIT IV ENZYMATIC AND BIOCHEMICAL ACTIVITIES OF BACTERIA 20 CATALASE TEST 21 OXIDASE TEST 22 COAGULASE TEST 23 UREASE TEST 24 INDOLE TEST 25 METHYL RED TEST 26 VOGES-PROSKAUER TEST 27 CITRATE UTILIZATION TEST 28 TRIPLE SUGAR IRON AGAR (TSI) TEST 29 HYDROGEN SULPHIDE TEST 30 NITRATE REDUCTION TEST UNIT V ANTIMICROBIAL SENSITIVITY TESTS 31 KIRBY-BAUER METHOD 32 STOKE’S METHOD 33 AGAR DILUTION METHOD 34 BROTH DILUTION METHOD 35 EPSILOMETER TEST (E-TEST) UNIT VI IMMUNOLOGY 36 BACTERIAL AGGLUTINATION TEST 37 BLOOD GROUPING 38 LATEX AGGLUTINATION TEST 39 CO-AGGLUTINATION TEST 40 WIDAL TEST 41 WEIL FELIX TEST 42 ANTI-STREPTOLYSIN O (ASLO) TEST 43 VDRL TEST 44 RADIAL IMMUNODIFFUSION TEST 45 IMMUNOELECTROPHORESIS TEST 46 COUNTER-CURRENT IMMUNOELECTROPHORESIS TEST 47 INDIRECT HAEMAGGLUTINATION TEST 48 IMMUNOFLUORESCENCE TEST 49 ENZYME-LINKED IMMUNOSORBENT ASSAY UNIT VII MICROBIAL GENETICS AND MOLECULAR TECHNIQUES 50 ISOLATION OF PLASMIDS 51 POLYACRYLAMIDE GEL ELECTROPHORESIS 52 ISOLATION OF ANTIBIOTIC RESISTANT MUTANT 53 BACTERIAL CONJUGATION UNIT VIII BACTERIOLOGY 54 NORMAL MICROBIAL FLORA OF THE MOUTH 55 NORMAL MICROBIAL FLORA OF THE THROAT 56 NORMAL MICROBIAL FLORA OF THE SKIN 57 IDENTIFICATION OF STAPHYLOCOCCUS AUREUS 58 IDENTIFICATION OF STREPTOCOCCUS PNEUMONIAE 59 IDENTIFICATION OF -HAEMOLYTIC STREPTOCOCCI 60 IDENTIFICATION OF CORYNEBACTERIUM DIPHTHERIAE 61 IDENTIFICATION OF LACTOSE FERMENTING ENTEROBACTERIACAE 62 IDENTIFICATION OF VIBRIO CHOLERAE 63 IDENTIFICATION OF PSEUDOMONAS AERUGINOSA UNIT IX PARASITOLOGY 64 SALINE WET MOUNT OF STOOL 65 IODINE WET MOUNT OF STOOL 66 LPCB WET MOUNT OF STOOL 67 ACID-FAST STAINING OF STOOL SMEARS 68 LEISHMAN’S STAINING OF PERIPHERAL BLOOD SMEARS 69 CONCENTRATION OF STOOL FOR PARASITES 70 CULTURE OF STOOL FOR ENTAMOEBA HISTOLYTICA UNIT X MYCOLOGY 71 CULTIVATION OF FUNGI 72 GRAM’S STAINING FOR FUNGI 73 LACTOPHENOL COTTON BLUE (LPCB) WET MOUNT OF FUNGI 74 POTASSIUM HYDROXIDE WET MOUNT OF FUNGI 75 INDIAN INK WET MOUNT PREPARATION 76 SLIDE CULTURE 77 GERM TUBE TEST 78 UREASE TEST 79 CARBOHYDRATE ASSIMILATION TEST 80 CARBOHYDRATE FERMENTATION TEST 81 IDENTIFICATION OF COMMON FUNGI UNIT XI VIROLOGY 82 CULTIVATION OF VIRUSES IN THE CELL LINES 83 CULTIVATION OF VIRUSES IN EMBRYONATED EGG UNIT XII MICROBIOLOGY OF WATER, MILK AND AIR 84 MICROBIOLOGY OF WATER 85 MICROBIOLOGY OF MILK 86 MICROBIOLOGY OF AIR UNIT XIII ANIMAL EXPERIMENTS 87 INTRAVENOUS INOCULATION INTO MICE TAIL VEIN 88 COLLECTION OF BLOOD FROM THE MARGINAL EAR VEIN OF RABBIT 89 ANIMALS AND THEIR USES IN THE LABORATORY UNIT XIV MEDICAL ENTOMOLOGY 90 IDENTIFICATION OF COMMON INSECTS UNIT XV COMMON VIVA SPOTS 91 IDENTIFICATION OF COMMON VIVA SPOTS