CELL MIGRATION IN INFLAMMATION AND IMMUNITY: METHODS AND PROTOCOLS
1 CHEMOTAXIS AND INTERACTION WITH VASCULAR OR LYMPHATIC ENDOTHELIUM 2 ANALYSIS OF INTEGRIN-DEPENDENT RAPID ADHESION UNDER LAMINAR-FLOW CONDITIONS 3 POSTTRANSLATIONAL PROCESSING OF CHEMOKINES 4 CHEMOTACTIC PROFILING OF LYMPHOCYTE SUBPOPULATIONS 5 MEASUREMENT OF THE LEVELS OF POLYMERIZED ACTIN (F-ACTIN) IN CHEMOKINE-STIMULATED LYMPHOCYTES AND GFP-COUPLED CDNA TRANSFECTED LYMPHOID CELLS BY FLOW CYTOMETRY 6 EVALUATION OF RHO FAMILY SMALL G-PROTEIN ACTIVITY INDUCED BY INTEGRIN LIGATION ON HUMAN LEUKOCYTES 7 RECONSTRUCTING LEUKOCYTE MIGRATION IN 3D EXTRACELLULAR MATRIX BY TIME-LAPSE VIDEOMICROSCOPY AND COMPUTER-ASSISTED TRACKING 8 ANALYZING CHEMOTAXIS USING DICTYOSTELIUM DISCOIDEUM AS A MODEL SYSTEM 9 CONDITIONAL TRANSGENIC MODELS TO STUDY CHEMOKINE BIOLOGY 10 INTRAVITAL MICROSCOPY AS A TOOL FOR STUDYING RECRUITMENT AND CHEMOTAXIS CONTENTS 11 TRACKING ANTIGEN-SPECIFIC LYMPHOCYTES IN VIVO 12 ANALYSIS OF HOMING-RECEPTOR EXPRESSION ON INFILTRATING LEUKOCYTES IN DISEASE STATES 13 INTERACTION OF VIRAL CHEMOKINE INHIBITORS WITH CHEMOKINES 14 DISCOVERY OF SMALL-MOLECULE ANTAGONISTS OF CHEMOKINE RECEPTORS 15 VISUALIZATION AND ANALYSIS OF ADHESIVE EVENTS IN BRAIN MICROVESSELS BY USING INTRAVITAL MICROSCOPY 16 ANIMAL MODELS TO STUDY CHEMOKINE RECEPTOR FUNCTION IN VIVO 17 ASSESSING THE ROLE OF MULTIPLE PHOSPHOINOSITIDE 3-KINASES IN CHEMOKINE SIGNALING 18 IN VITRO AND IN VIVO MODELS TO STUDY CHEMOKINE REGULATION OF ANGIOGENESIS 19 REAL-TIME IN VITRO ASSAY FOR STUDYING CHEMOATTRACTANT- TRIGGERED LEUKOCYTE TRANSENDOTHELIAL MIGRATION UNDER PHYSIOLOGICAL FLOW CONDITIONS 20 GENERATION OF MONOCLONAL ANTIBODIES AGAINST CHEMOKINE RECEPTORS 21 DETECTION OF HIGH-AFFINITY ?4-INTEGRIN UPON LEUKOCYTE STIMULATION BY CHEMOATTRACTANTS OR CHEMOKINES