ADVANCES IN GENETICS

By C. JOANI

PART I MOUSE GENETICS TECHNIQUES 1 DEVELOPMENT OF PRONUCLEAR INJECTION-BASED TARGETED TRANSGENESIS IN MICE THROUGH CRE–LOXP SITE-SPECIFIC RECOMBINATION 2 GENERATION OF CONDITIONAL KNOCKOUT MICE 3 GENERATING MOUSE LINES FOR LINEAGE TRACING AND KNOCKOUT STUDIES 4 GENERATION OF AN ALLELIC SERIES OF KNOCK-IN MICE USING RECOMBINASE-MEDIATED CASSETTE EXCHANGE (RMCE) 5 GENERATING CHIMERIC MICE FROM EMBRYONIC STEM CELLS VIA VIAL COCULTURING OR HYPERTONIC MICROINJECTION 6 GENETIC INDUCIBLE FATE MAPPING IN ADULT MICE USING TAMOXIFEN-DEPENDENT CRE RECOMBINASES 7 INTRACYTOPLASMIC SPERM INJECTION (ICSI)-MEDIATED TRANSGENESIS IN MICE 8 GENERATION OF BACTERIAL ARTIFICIAL CHROMOSOME (BAC) TRANSGENIC MICE 9 GENERATION OF GENETICALLY ENGINEERED MICE BY THE PIGGYBAC TRANSPOSON SYSTEM 10 GENERATION AND APPLICATIONS OF MADM-BASED MOUSE GENETIC MOSAIC SYSTEM 11 GENERATION OF MOUSE LINES CONDITIONALLY OVER-EXPRESSING MICRORNA USING THE ROSA26-LOX-STOP-LOX SYSTEM 12 IN SITU HYBRIDIZATION (BOTH RADIOACTIVE AND NONRADIOACTIVE) AND SPATIOTEMPORAL GENE EXPRESSION ANALYSIS CONTENTS PART II MOUSE GENETICS IN STEM CELLS 13 ISOLATION AND HANDLING OF MOUSE EMBRYONIC FIBROBLASTS 14 GENERATION OF INDUCED PLURIPOTENT STEM CELLS FROM MOUSE ADIPOSE TISSUE 15 PDX1 (GFP/W) MICE FOR ISOLATION, CHARACTERIZATION, AND DIFFERENTIATION OF PANCREATIC PROGENITOR CELLS 16 GENERATION OF TRANSGENIC MOUSE FLUORESCENT REPORTER LINES FOR STUDYING HEMATOPOIETIC DEVELOPMENT 17 ANALYZING GENE FUNCTION IN ADULT LONG-TERM HEMATOPOIETIC STEM CELLS USING THE INTERFERON INDUCIBLE MX1-CRE MOUSE SYSTEM 18 GENERATION OF TRANSGENIC MICE BY EXPLOITING SPERMATOGONIAL STEM CELLS IN VIVO PART III MOUSE GENETICS IN MODELING HUMAN DISEASE 19 METHODS FOR THE DETECTION OF GENOME INSTABILITY DERIVED FROM REPLICATION STRESS IN PRIMARY MOUSE EMBRYONIC FIBROBLASTS 20 TRANSGENIC NUDE MICE UBIQUITOUSLY EXPRESSING FLUORESCENT PROTEINS FOR COLOR-CODED IMAGING OF THE TUMOR MICROENVIRONMENT 21 GENETICALLY ENGINEERED INSERTIONAL MUTAGENESIS IN MICE TO MODEL CANCER: SLEEPING BEAUTY 22 GENERATING DOUBLE KNOCKOUT MICE TO MODEL GENETIC INTERVENTION FOR DIABETIC CARDIOMYOPATHY IN HUMANS 23 EXPERIMENTAL OSTEOARTHRITIS MODELS IN MICE 24 MOUSE MODELS AND METHODS FOR STUDYING HUMAN DISEASE, ACUTE KIDNEY INJURY (AKI) 25 MOUSE MODELS OF ACUTE AND CHRONIC COLITIS 26 MANIPULATION AND ASSESSMENT OF GUT MICROBIOME FOR METABOLIC STUDIES