IN SITU HYBRIDIZATION

By Q. MAANY

CONTENTS PART 1 CHROMOSOMAL TECHNIQUES PREPARATION OF HUMAN PARTIAL CHROMOSOME PAINTS FROM SOMATIC CELL HYBRIDS DNA–PROTEIN IN SITU COVISUALIZATION FOR CHROMOSOME ANALYSIS RADIOACTIVE IN SITU HYBRIDIZATION TO ANIMAL CHROMOSOMES NATIVE POLYTENE CHROMOSOMES OF DROSOPHILA MELANOGASTER FOR LIGHT AND ELECTRON MICROSCOPIC OBSERVATION OF THE CONFORMATION AND DISTRIBUTION OF MOLECULES RELEASED CHROMATIN OR DNA FIBER PREPARATIONS FOR HIGH-RESOLUTION FIBER FISH IN SITU HYBRIDIZATION TO POLYTENE CHROMOSOMES OF DROSOPHILA MELANOGASTER AND OTHER DIPTERAN SPECIES IN SITU HYBRIDIZATION TO POLYTENE CHROMOSOMES COMPARATIVE GENE MAPPING IN EXOTIC SPECIES USING FISH PART 2 TISSUE TECHNIQUES IN SITU HYBRIDIZATION WITH NONRADIOACTIVE PROBES 10 QUANTITATIVE IN SITU HYBRIDIZATION USING RADIOACTIVE PROBES TO STUDY GENE EXPRESSION IN HETEROCELLULAR SYSTEMS 11 IN SITU END-LABELING OF FRAGMENTED DNA AND THE LOCALIZATION OF APOPTOSIS 12 COMBINED IMMUNOHISTOCHEMICAL LABELING AND IN SITU HYBRIDIZATION TO COLOCALIZE MRNA AND PROTEIN IN TISSUE SECTIONS 13 IN SITU HYBRIDIZATION PROTOCOLS FOR DETECTION OF VIRAL DNA USING RADIOACTIVE AND NONRADIOACTIVE DNA PROBES 14 SIGNAL AMPLIFICATION FOR DNA AND MRNA 15 IN SITU LOCALIZATION OF PCR-AMPLIFIED DNA AND CDNA 16 ULTRASTRUCTURAL DETECTION OF NUCLEIC ACIDS ON THIN SECTIONS OF TISSUE EMBEDDED IN HYDROPHILIC RESIN 17 IN SITU HYBRIDIZATION FOR ELECTRON MICROSCOPY 18 IN SITU HYBRIDIZATION OF WHOLE-MOUNT EMBRYOS 19 CRNA PROBES: COMPARISON OF ISOTOPIC AND NONISOTOPIC DETECTION METHODS PART 3 APPLICATIONS 20 MOLECULAR CYTOGENETIC ANALYSIS OF SPERM FROM INFERTILE MALES UNDERGOING INTRACYTOPLASMIC SPERM INJECTION 21 LOCALIZATION OF HIV-1 DNA AND TUMOR NECROSIS FACTOR-? MRNA IN HUMAN BRAIN USING POLYMERASE CHAIN REACTION IN SITU HYBRIDIZATION AND IMMUNOCYTOCHEMISTRY