PAPER CHROMATOGRAPHY

By JOSHUA ANDERSON

CONTENTS AN OVERVIEW OF AFFINITY CHROMATOGRAPHY WEAK AFFINITY CHROMATOGRAPHY FLUIDIZED-BED RECEPTOR–AFFINITY CHROMATOGRAPHY SITE-SPECIFIC IMMOBILIZATION OF ANTIBODIES TO PROTEIN G-DERIVATIZED SOLID SUPPORTS AFFINITY PURIFICATION OF MONOCLONAL ANTIBODIES PROTEIN A MIMETIC (PAM) AFFINITY CHROMATOGRAPHY: IMMUNOGLOBULINS PURIFICATION PERIODATE OXIDATION OF ANTIBODIES FOR SITE-SELECTIVE IMMOBILIZATION IN IMMUNOAFFINITY CHROMATOGRAPHY MINI-ANTIBODY AFFINITY CHROMATOGRAPHY OF LYSOZYME NITROCELLULOSE-BASED IMMUNOAFFINITY CHROMATOGRAPHY IMMUNOAFFINITY CHROMATOGRAPHY AFFINITY PARTITIONING OF PROTEINS BORONATE AFFINITY CHROMATOGRAPHY DYE–LIGAND AFFINITY CHROMATOGRAPHY FOR PROTEIN SEPARATION AND PURIFICATION DNA AFFINITY CHROMATOGRAPHY AFFINITY CHROMATOGRAPHY OF PYROGENS WESTERN CROSS BLOT AFFINITY PERFUSION CHROMATOGRAPHY PHAGE DISPLAY TECHNOLOGY: AFFINITY SELECTION BY BIOPANNING PHAGE DISPLAY TECHNOLOGY: IDENTIFICATION OF PEPTIDES AS MODEL LIGANDS FOR AFFINITY CHROMATOGRAPHY AN OVERVIEW OF AFFINITY CHROMATOGRAPHY 1 INTRODUCTION AFFINITY CHROMATOGRAPHY IS PERVASIVELY ACCEPTED AND USED AS A TOOL IN BIO- MEDICAL RESEARCH AND BIOTECHNOLOGY; YET ITS ORIGINS ONLY 30 YEARS AGO SOME- TIMES SEEM DIMMED IN HISTORY HOWEVER, THE POTENTIAL OF THIS TECHNOLOGY CONTINUES TO STIMULATE CONTINUED DEVELOPMENT AND NEW APPLICATIONS HAVING A NEW BOOK ON THIS METHODOLOGY IS EMINENTLY APPROPRIATE TODAY AND BEING ABLE TO INTRODUCE THIS BOOK IS OUR PLEASURE AFFINITY CHROMATOGRAPHY AS IT IS KNOWN TODAY WAS INTRODUCED IN 1968 BY PEDRO CUATRECASAS, CHRIS ANFINSEN, AND MEIR WILCHEK, ONE OF THE AUTHORS OF THIS CHAPTER THOUGH FEW RELATED METHODS WERE DESCRIBED EARLIER, THE CONCEPT AND IMMENSE POWER OF BIORECOGNITION AS A MEANS OF PURIFICATION WAS INTRO- DUCED FIRST IN THAT 1968 PAPER (1) ENTITLED “AFFINITY CHROMATOGRAPHY” IF YOU EXAMINE THE MEDLINE DATABASE FOR HOW MANY TIMES “AFFINITY CHROMA- TOGRAPHY” HAS APPEARED IN THE TITLE OF SCIENTIFIC PAPERS, YOU WILL FIND ALMOST 30,000 PAPERS CITED THIS MEANS THAT, OVER THE PAST 30 YEARS, THREE PUBLISHED PAPERS PER DAY HAVE FEATURED THIS TECHNOLOGY MOREOVER, 300 PATENTS HAVE BEEN GRANTED DURING THE LAST 2 YEARS ALONE IN A RECENT REVIEW (2), CHRIS LOWE STATED THAT AFFINITY CHROMATOGRAPHY IS A TECHNIQUE USED IN 60% OF ALL PURIFICATION PROTOCOLS SO WHAT EXACTLY IS AFFINITY CHROMATOGRAPHY—THE TECHNIQUE TO WHICH THIS BOOK IS DEVOTED? 2 AFFINITY CHROMATOGRAPHY AND ITS APPLICATIONS FOR PURIFICATION AFFINITY CHROMATOGRAPHY IS BASED ON MOLECULAR RECOGNITION IT IS A RELATIVELY SIMPLE PROCEDURE ANY GIVEN BIOMOLECULE THAT ONE WISHES TO PURIFY USUALLY HAS AN INHERENT RECOGNITION SITE THROUGH WHICH IT CAN RECOGNIZE A NATURAL OR ARTIFI- CIAL MOLECULE IF ONE OF THESE RECOGNITION PARTNERS IS IMMOBILIZED ON A POLY