METHODS BASED ON POLARITY
1 Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations 1 2 Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell 19 3 Protein–Protein Interaction Techniques: Dissect PCP Signaling in Xenopus 27 4 Cuticle Refraction Microscopy: A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity 43 5 Analysis of Cell Shape and Polarity During Zebrafish Gastrulation 53 6 Analyzing Planar Cell Polarity During Zebrafish Gastrulation 69 7 Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements During Xenopus Gastrulation 79 8 Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling 91 9 Gene Loss-of-Function and Live Imaging in Chick Embryos 105 10 Activation and Function of Small GTPases Rho, Rac, and Cdc42 During Gastrulation 119 11 Convergent Extension Analysis in Mouse Whole Embryo Culture 133 12 Analysis of PCP Defects in Mammalian Eye Lens 147 13 Examining Planar Cell Polarity in the Mammalian Cochlea 157 14 Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells 173 15 The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration 187 16 Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells 201 17 Regulation of Focal Adhesion Dynamics by Wnt5a Signaling 215 18 The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity 229 19 Assessing PCP in the Cochlea of Mammalian Ciliopathy Models 239 20 Morphometric Analysis of Centrosome Position in Tissues 249