NATURAL KILLER CELL PROTOCOLS

By SUSAN RAMIREZ

1 CLONING HUMAN NATURAL KILLER CELLS 2 GENERATION OF SHORT-TERM MURINE NATURAL KILLER CELL CLONES TO ANALYZE LY49 GENE EXPRESSION 4 TECHNIQUES FOR STUDYING DEVELOPMENT OF HUMAN NATURAL KILLER CELLS AND T CELLS 5 MURINE NATURAL KILLER CELL CLONING FROM FETAL THYMIC ORGAN CULTURES 6 GENERATION OF HLA CLASS I TRANSFECTED TARGET CELL LINES 7 MURINE NATURAL KILLER CELLS AND HYBRID RESISTANCE TO HEMOPOIETIC CELLS IN VIVO 8 HYBRID RESISTANCE BY MOUSE NK CELLS IN VITRO 9 USE OF ACTIVATED NATURAL KILLER CELLS FOR TUMOR IMMUNOTHERAPY IN MOUSE AND HUMAN 10 METHODS FOR IN VIVO ANALYSES OF NATURAL KILLER (NK) CELLS 11 ASSAY FOR THE TRANSENDOTHELIAL MIGRATION OF HUMAN NATURAL KILLER CELLS 12 METHODS OF ANALYZING CHROMATIN CHANGES ACCOMPANYING APOPTOSIS OF TARGET CELLS IN KILLER CELL ASSAYS CONTENTS 13 METHODS FOR DETECTING LYTIC GRANULES IN NATURAL KILLER CELLS 14 FLUOROGENIC SUBSTRATES AS DETECTORS OF CASPASE ACTIVITY DURING NATURAL KILLER CELL-INDUCED APOPTOSIS 15 TECHNIQUES FOR STUDYING MURINE NATURAL KILLER CELLS IN DEFENSE AGAINST VIRAL INFECTION 16 ASSAYS FOR ANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXICITY (ADCC) AND REVERSE ADCC (REDIRECTED CYTOTOXICITY) IN HUMAN NATURAL KILLER CELLS 18 GENERATION OF ANTIBODIES TO CELL SURFACE MARKERS ON MATURE NATURAL KILLER CELLS 19 HUMAN SINGLE-CHAIN FV FRAGMENTS SPECIFIC FOR NATURAL KILLER CELL RECEPTORS FROM PHAGE DISPLAY LIBRARIES 20 BINDING OF SOLUBLE KIR-FC FUSION PROTEINS TO HLA CLASS I 21 GENETIC ANALYSIS OF A HIGHLY HOMOLOGOUS GENE FAMILY 22 USE OF VACCINIA VIRUS FOR FUNCTIONAL GENE TRANSFER IN NATURAL KILLER CELLS 23 USE OF CDNA LIBRARY EXPRESSION CLONING TO IDENTIFY COMPONENTS OF HETERODIMERIC RECEPTOR COMPLEXES 24 FUNCTIONAL ANALYSIS OF NATURAL KILLER CELL RECEPTORS IN THE RNK-16 RAT LEUKEMIC CELL LINE 25 USE OF MUTANT JURKAT T CELL LINES TO DEFINE HUMAN NATURAL KILLER CELL SURFACE RECEPTOR SIGNALING PATHWAYS USE OF MUTANT JURKAT T CELL LINES TO DEFINE HUMAN NATURAL KILLER CELL SURFACE RECEPTOR SIGNALING PATHWAYS DOMAIN INTERACTIONS 28 ANALYSIS AND USE OF MUTANT MICE EXHIBITING NATURAL KILLER CELL DEFECTS 29 ISOLATION AND ANALYSIS OF NATURAL KILLER CELLS IN CHICKENS 30 ANALYZING NONSPECIFIC CYTOTOXIC CELLS IN FISH 31 NATURAL KILLER CELLS AND IMMUNOTOXICOLOGY