A handbook of PCR
CONTENTS PART I PERFORMING AND OPTIMIZING PCR POLYMERASE CHAIN REACTION: BASIC PRINCIPLES AND ROUTINE PRACTICE COMPUTER PROGRAMS FOR PCR PRIMER DESIGN AND ANALYSIS SINGLE-STEP PCR OPTIMIZATION USING TOUCHDOWN AND STEPDOWN PCR PROGRAMMING XL PCR AMPLIFICATION OF LONG TARGETS FROM GENOMIC DNA COUPLED ONE-STEP REVERSE TRANSCRIPTION AND POLYMERASE CHAIN REACTION PROCEDURE FOR CLONING LARGE CDNA FRAGMENTS LONG DISTANCE REVERSE-TRANSCRIPTION PCR INCREASING PCR SENSITIVITY FOR AMPLIFICATION FROM PARAFFIN-EMBEDDED TISSUES GC-RICH TEMPLATE AMPLIFICATION BY INVERSE PCR: DNA POLYMERASE AND SOLVENT EFFECTS PCR PROCEDURE FOR THE ISOLATION OF TRINUCLEOTIDE REPEATS METHYLATION-SPECIFIC PCR DIRECT CLONING OF FULL-LENGTH CELL DIFFERENTIALLY EXPRESSED GENES BY MULTIPLE ROUNDS OF SUBTRACTIVE HYBRIDIZATION BASED ON LONG-DISTANCE PCR AND MAGNETIC BEADS PART II CLONING PCR PRODUCTS CLONING PCR PRODUCTS: AN OVERVIEW USING T4 DNA POLYMERASE TO GENERATE CLONABLE PCR PRODUCTS ENZYME-FREE CLONING OF PCR PRODUCTS AND FUSION PROTEIN EXPRESSION DIRECTIONAL RESTRICTION SITE-FREE INSERTION OF PCR PRODUCTS INTO VECTORS AUTOSTICKY PCR: DIRECTIONAL CLONING OF PCR PRODUCTS WITH PREFORMED 5' OVERHANGS A RAPID AND SIMPLE PROCEDURE FOR DIRECT CLONING OF PCR PRODUCTS INTO BACULOVIRUSES PART III MUTAGENESIS AND RECOMBINATION PCR APPROACHES TO DNA MUTAGENESIS AND RECOMBINATION: AN OVERVIEW IN-FRAME CLONING OF SYNTHETIC GENES USING PCR INSERTS MEGAPRIMER PCR PCR-MEDIATED RECOMBINATION: A GENERAL METHOD APPLIED TO CONSTRUCT CHIMERIC INFECTIOUS MOLECULAR CLONES PCR METHOD FOR GENERATING MULTIPLE MUTATIONS AT ADJACENT SITES A FAST POLYMERASE CHAIN REACTION-MEDIATED STRATEGY FOR INTRODUCING REPEAT EXPANSIONS INTO CAG-REPEAT CONTAINING GENES PCR SCREENING IN SIGNATURE-TAGGED MUTAGENESIS OF ESSENTIAL GENES STAGGERED EXTENSION PROCESS (STEP) IN VITRO RECOMBINATION RANDOM MUTAGENESIS BY WHOLE-PLASMID PCR AMPLIFICATION PART IV CLONING UNKNOWN NEIGHBORING DNA PCR-BASED STRATEGIES TO CLONE UNKNOWN DNA REGIONS FROM KNOWN FOREIGN INTEGRANTS: AN OVERVIEW LONG DISTANCE VECTORETTE PCR (LDV PCR) NONSPECIFIC, NESTED SUPPRESSION PCR METHOD FOR ISOLATION OF UNKNOWN FLANKING DNA (“COLD-START METHOD”) INVERSE PCR: CDNA CLONING INVERSE PCR: GENOMIC DNA CLONING GENE CLONING AND EXPRESSION PROFILING BY RAPID AMPLIFICATION OF GENE INSERTS WITH UNIVERSAL VECTOR PRIMERS THE ISOLATION OF DNA SEQUENCES FLANKING TN5 TRANSPOSON INSERTIONS BY INVERSE PCR RAPID AMPLIFICATION OF GENOMIC DNA SEQUENCES TAGGED BY INSERTIONAL MUTAGENESIS ISOLATION OF LARGE TERMINAL SEQUENCES OF BAC INSERTS BASED ON DOUBLE-RESTRICTION-ENZYME DIGESTION FOLLOWED BY ANCHORED PCR A “STEP DOWN” PCR-BASED TECHNIQUE FOR WALKING INTO AND THE SUBSEQUENT DIRECT SEQUENCE ANALYSIS OF FLANKING GENOMIC DNA PART V LIBRARY CONSTRUCTION AND SCREENING USE OF PCR IN LIBRARY SCREENING: AN OVERVIEW CLONING OF HOMOLOGOUS GENES BY GENE-CAPTURE PCR RAPID AND NONRADIOACTIVE SCREENING OF RECOMBINANT LIBRARIES BY PCR RAPID CDNA CLONING BY PCR SCREENING (RC-PCR) GENERATION AND PCR SCREENING OF BACTERIOPHAGE ? SUBLIBRARIES ENRICHED FOR RARE CLONES (THE “SUBLIBRARY METHOD”) PCR-BASED SCREENING FOR BACTERIAL ARTIFICIAL CHROMOSOME LIBRARIES A 384-WELL MICROTITER-PLATE-BASED TEMPLATE PREPARATION AND SEQUENCING METHOD A MICROTITER-PLATE-BASED HIGH THROUGHPUT PCR PRODUCT PURIFICATION METHOD