TIME, CELLS, AND AGING
CONTENTS PART I CONCEPTS 1 PROTEIN MISFOLDING AND CELLULAR STRESS: AN OVERVIEW 2 PROTEIN AGGREGATION DISEASES: TOXICITY OF SOLUBLE PREFIBRILLAR AGGREGATES AND THEIR CLINICAL SIGNIFICANCE 3 CONSEQUENCES OF STRESS IN THE SECRETARY PATHWAY: THE ER STRESS RESPONSE AND ITS ROLE IN THE METABOLIC SYNDROME 4 WHAT ROLE DOES MITOCHONDRIAL STRESS PLAY IN NEURODEGENERATIVE DISEASES? 5 AUTOPHAGY IN DISEASE 6 MITOPHAGY AND MITOPTOSIS IN DISEASE PROCESSES 7 CELLULAR STRESS AND PROTEIN MISFOLDING DURING AGING 8 MEASURING CONSEQUENCES OF PROTEIN MISFOLDING AND CELLULAR STRESS USING OMICS TECHNIQUES PART II PROTOCOLS 9 PRODUCTION OF CELLS WITH TARGETED INTEGRATION OF GENE VARIANTS OF HUMAN ABC TRANSPORTER FOR STABLE AND REGULATED EXPRESSION USING THE FLP RECOMBINASE SYSTEM 10 STRESS RESPONSE PROFILES IN HUMAN FIBROBLASTS EXPOSED TO HEAT SHOCK OR OXIDATIVE STRESS 11 EXAMINING UBIQUITINATED PROTEIN AGGREGATES IN TISSUE SECTIONS 12 DETERMINATION OF PROTEASOMAL ACTIVITIES 13 MEASUREMENT OF AUTOPHAGY IN CELLS AND TISSUES 14 TRANSCRIPTION FACTOR SEQUESTRATION BY POLYGLUTAMINE PROTEINS 15 BIOLOGICAL MEMBRANES AS PROTEIN AGGREGATION MATRICES AND TARGETS OF AMYLOID TOXICITY 16 MEASUREMENT OF MITOCHONDRIAL ROS PRODUCTION 17 IN VIVO DETECTION OF OXIDIZED PROTEINS: A PRACTICAL APPROACH TO TISSUE-DERIVED MITOCHONDRIA 18 MEASUREMENT OF OXIDIZED/REDUCED GLUTATHIONE RATIO 19 DETERMINATION OF ALTERED MITOCHONDRIA ULTRASTRUCTURE BY ELECTRON MICROSCOPY 20 ASSESSING BAD SUB-CELLULAR LOCALIZATION UNDER CONDITIONS ASSOCIATED WITH PREVENTION OR PROMOTION OF MITOCHONDRIAL PERMEABILITY TRANSITION-DEPENDENT TOXICITY 21 A CELLULAR VIABILITY ASSAY TO MONITOR DRUG TOXICITY 22 RESCUE OF MISFOLDED PROTEINS AND STABILIZATION BY SMALL MOLECULES