LABORATORY DATABASE MANAGEMENT
CONTENTS 1 INTRODUCTION: STRATEGIES FOR DEVELOPING GENETICALLY MODIFIED MICE 2 GENETIC MODIFICATION OF THE MOUSE: GENERAL TECHNOLOGY – PRONUCLEAR AND BLASTOCYST INJECTION 3 GENERATION OF CHIMERAS BY AGGREGATION OF EMBRYONIC STEM CELLS WITH DIPLOID OR TETRAPLOID MOUSE EMBRYOS 4 CRYOPRESERVATION OF MOUSE SPERMATOZOA AND IN VITRO FERTILIZATION 5 AUTOPSY AND HISTOLOGICAL ANALYSIS OF THE TRANSGENIC MOUSE 6 TRANSGENE DESIGN 7 INDUCIBLE TRANSGENIC MOUSE MODELS 8 LENTIVIRAL TRANSGENESIS 9 TRANSGENESIS IN MOUSE EMBRYONIC STEM CELLS 10 ENGINEERING BAC REPORTER GENE CONSTRUCTS FOR MOUSE TRANSGENESIS 11 TARGETING VECTOR CONSTRUCTION THROUGH RECOMBINEERING 12 GENERATING CONDITIONAL KNOCKOUT MICE 13 HYPOMORPHIC MICE 14 MICER TARGETING VECTORS FOR MANIPULATING THE MOUSE GENOME 15 KNOCK-IN APPROACHES 16 GENERATION OF A SERIES OF KNOCK-IN ALLELES USING RMCE IN ES CELLS 17 SELECTION OF TARGETED MUTANTS FROM A LIBRARY OF RANDOMLY MUTAGENIZED ES CELLS 18 GENERATION OF GENETICALLY MODIFIED RODENTS USING RANDOM ENU MUTAGENESIS 19 BONE MARROW TRANSPLANTATIONS TO STUDY GENE FUNCTION IN HEMATOPOIETIC CELLS 20 ADENOVIRUS-MEDIATED GENE TRANSFER