ANALYTICAL MOLECULAR BIOLOGY

By C. CAINU

CONTENTS CHAPTER 1 ANTIBODIES BINDING ANTIGENS VACCINATION CHIMERIC ANTIBODIES HUMANIZING MOUSE ANTIBODIES HUMAN ANTIBODIES IN MOUSE PHAGE DISPLAY LIBRARY FOLDING OF CDR’S AND PREDICTION OF ANTIBODY COMBINING SITES ANTIBODY DESIGN APPLICATION TO OTHER PROBLEMS IN MOLECULAR BIOLOGY REFERENCES EXERCISE CHAPTER 2 SATURATION OF HEMOGLOBIN WITH OXYGEN AT EQUILIBRIUM INTRODUCTION STRUCTURES OF MYOGLOBIN AND HEMOGLOBIN EQUILIBRIUM BINDING OF MYOGLOBIN WITH OXYGEN EXPERIMENTAL MEASUREMENTS OF SATURATION OF HEMOGLOBIN WITH OXYGEN AT EQUILIBRIUM ONE-CONSTANT MODEL TWO-CONSTANT MODEL THREE-CONSTANT MODEL LEAST SQUARE FITTING PREDICTION BASED ON THESE CONSTANTS FURTHER EXPERIMENTAL STUDIES OTHER STUDIES ON HEMOGLOBIN 2,3-DIPHOSPHOGLYCERATE OTHER ALLOSTERIC PROTEINS AND ENZYMES REFERENCES EXERCISE CHAPTER 3 CO-TRANSDUCTION AND LOCATION OF ESCHERICHIA COLI GENETIC MARKERS INTRODUCTION CONJUGATION TRANSDUCTION MODEL FOR RANDOM GENERALIZED TRANSDUCTION THREE-POINT ANALYSIS AN EXAMPLE ESCHERICHIA COLI GENETIC MAP FUTURE WORK REFERENCES EXERCISE CHAPTER 4 ENZYME KINETICS INTRODUCTION SIMPLIFIED REACTION RATE EQUATIONS SIMPLE ENZYME KINETICS TRANSITION REGION LOW SUBSTRATE CONCENTRATION NUMERICAL SOLUTION FOR NON-LINEAR RATE EQUATIONS INHIBITORS FUTURE STUDIES REFERENCES EXERCISE CHAPTER 5 THE AND ANGLES OF PROTEINS INTRODUCTION THE AND ANGLES PRECISE DEFINITION OF THE ANGLE PRECISE DEFINITION OF THE ANGLE PROTEIN DATA BANK UNIT NORMALS TO PLANES AND ANGLES AS ANGLES BETWEEN PLANES AN EXAMPLE BOND DISTANCES AND BOND ANGLES RAMACHANDRAN PLOT AND STERIC HINDRANCE ALLOWED CONFIGURATIONS OF AMINO ACID RESIDUES CALCULATIONS OF COORDINATES OF NON-BONDING ATOMS