CLONING NEUROPEPTIDE TYROSINE CDNA
CONTENTS PART I CLONING TECHNIQUES CLONING NEUROPEPTIDE TYROSINE CDNA HUMAN Y1/Y5 RECEPTOR GENE CLUSTER: ISOLATION AND CHARACTERIZATION HUMANTYPE 2 NEUROPEPTIDE Y RECEPTOR GENE: ISOLATION AND CHARACTERIZATION Y4 RECEPTOR IN DIFFERENT SPECIES: FUNCTIONAL EXPRESSION AND BINDING NEUROPEPTIDE Y Y5 RECEPTOR EXPRESSION HOMOLOGY-BASED CLONING METHODS: IDENTIFICATION OF THE NPY Y2, Y4, AND Y6 RECEPTORS PART II PRODUCTION OF TRANSGENIC AND KNOCK-OUT MODELS DEVELOPING TRANSGENIC NEUROPEPTIDE Y RATS NEUROPEPTIDE Y Y1 RECEPTOR-DEFICIENT MICE: GENERATION AND CHARACTERIZATION PART III ANTISENSE TECHNOLOGY ANTISENSE OLIGONUCLEOTIDE APPROACH TO STUDY NPY-MEDIATED FEEDING SIGNAL TRANSDUCTION 10 A G_I RNA-ANTISENSE EXPRESSION STRATEGY TO INVESTIGATE COUPLING OF PEPTIDE YY/NEUROPEPTIDE Y RECEPTOR TO G_I 11 NPY Y5 RECEPTOR SUBTYPE: PHARMACOLOGICAL CHARACTERIZATION WITH ANTISENSE OLIGODEOXYNUCLEOTIDE SCREENING STRATEGY 12 NPY ANTISENSE OLIGODEOXYNUCLEOTIDES TO STUDY THE ACTIONS OF NPY PART IV TECHNIQUES TO STUDY TISSUE DISTRIBUTION OF MRNA 13 LOCALIZATION OF Y-RECEPTOR SUBTYPE MRNAS IN RAT BRAIN BY DIGOXIGENIN LABELED IN SITU HYBRIDIZATION 14 CENTRAL Y4 RECEPTOR DISTRIBUTION: RADIOACTIVE RIBONUCLEOTIDE PROBE IN SITU HYBRIDIZATION WITH IN VITRO RECEPTOR AUTORADIOGRAPHY 15 COMBINING NON-ISOTOPIC LOCALIZATION OF NPY MRNA WITH IMMUNOCYTOCHEMISTRY PART V MISCELLANEOUS TECHNIQUES 16 Y RECEPTORS CHARACTERIZED BY RT-PCR: DISTRIBUTION IN RAT INTESTINE 17 QUANTIFICATION OF NPY MRNA BY RIBONUCLEASE PROTECTION ASSAY 18 RADIOLIGAND BINDING STUDIES: PHARMACOLOGICAL PROFILES OF CLONED Y-RECEPTOR SUBTYPES