PEPTIDE RECOGNITION MECHANISMS OF EUKARYOTIC SIGNALING MODULES
CONTENTS PART I OVERVIEWS 1 TRANSMEMBRANE SIGNALING BY G PROTEIN-COUPLED RECEPTORS 2 CROSSTALK COREGULATION MECHANISMS OF G PROTEIN-COUPLED RECEPTORS AND RECEPTOR TYROSINE KINASES 3 PROTEIN–PROTEIN INTERACTIONS IN SIGNALING CASCADES PART II SPECIFIC TOPICS A MEMBRANE RECEPTORS AND SIGNALING 4 BIOLOGICAL ROLE OF THE CXCR4–SDF-1 AXIS IN NORMAL HUMAN HEMATOPOIETIC CELLS 5 FUNCTIONAL EXPRESSION OF CXCR4 IN SACCHAROMYCES CEREVISIAE IN THE DEVELOPMENT OF POWERFUL TOOLS FOR THE PHARMACOLOGICAL CHARACTERIZATION OF CXCR4 6 CHARACTERIZATION OF CONSTITUTIVELY ACTIVE MUTANTS OF G PROTEIN-COUPLED RECEPTORS 7 G PROTEIN-COUPLED RECEPTOR DIMERIZATION AND SIGNALING 8 REAL-TIME ANALYSIS OF G PROTEIN-COUPLED RECEPTOR SIGNALING IN LIVE CELLS B LIPID RAFT AND CAVEOLAE IN TRANSMEMBRANE SIGNALING 9 ISOLATION OF MEMBRANE RAFTS AND SIGNALING COMPLEXES 10 METHODS FOR THE STUDY OF SIGNALING MOLECULES IN MEMBRANE LIPID RAFTS AND CAVEOLAE C PROTEIN–PROTEIN INTERACTION IN TRANSMEMBRANE SIGNALING 11 BIOLUMINESCENCE RESONANCE ENERGY TRANSFER TO MONITOR PROTEIN–PROTEIN INTERACTIONS 12 IDENTIFICATION OF INTERACTING PROTEINS USING THE YEAST TWO-HYBRID SCREEN 13 ANALYSIS OF PDZ DOMAIN INTERACTIONS USING YEAST TWO-HYBRID AND COIMMUNOPRECIPITATION ASSAYS 14 CLUSTERING ASSAY FOR STUDYING THE INTERACTION OF MEMBRANE PROTEINS WITH PDZ DOMAIN PROTEINS D RHO GUANOSINE TRIPHOSPHATASES AND RECONSTITUTION OF SIGNALING COMPLEXES 15 MAMMALIAN CELL MICROINJECTION ASSAY TO STUDY THE FUNCTION OF RHO FAMILY GUANOSINE TRIPHOSPHATASES 16 AFFINITY-BASED ASSAY OF RHO GUANOSINE TRIPHOSPHATASE ACTIVATION 17 ASSAY OF PHOSPHOLIPASE D ACTIVITY IN CELL-FREE SYSTEMS 18 RECONSTITUTION SYSTEM BASED ON CYTOSOL-DEPLETED CELLS TO STUDY THE REGULATION OF PHOSPHOLIPASE D E GENOMICS AND PROTEOMICS 19 ANALYSIS OF GLOBAL GENE EXPRESSION PROFILES ACTIVATED BY CHEMOATTRACTANT RECEPTORS 20 GENETIC RECONSTITUTION OF BONE MARROW FOR THE STUDY OF SIGNAL TRANSDUCTION EX VIVO 21 PROTEOMIC ANALYSIS OF HUMAN NEUTROPHILS