BIOLOGY AT THE SINGLE-MOLECULE LEVEL

By W. LUARY

CONTENTS PART I GENERAL ASPECTS COMPARATIVE ANALYSIS OF FLUORESCENCE REPORTER SIGNALS BASED ON INTENSITY, ANISOTROPY, TIME-RESOLUTION, AND WAVELENGTH-RATIOMETRY PART II DESIGN OF ORGANIC DYES OPTIMIZED UV/VISIBLE FLUORESCENT MARKERS LONG-WAVELENGTH PROBES AND LABELS BASED ON CYANINES AND SQUARAINES TWO-PHOTON ABSORPTION IN NEAR-IR CONJUGATED MOLECULES: DESIGN STRATEGY AND STRUCTURE–PROPERTY RELATIONS DISCOVERY OF NEW FLUORESCENT DYES: TARGETED SYNTHESIS OR COMBINATORIAL APPROACH? PART III ORGANIC DYES WITH RESPONSE FUNCTION PHYSICAL PRINCIPLES BEHIND SPECTROSCOPIC RESPONSE OF ORGANIC FLUOROPHORES TO INTERMOLECULAR INTERACTIONS ORGANIC DYES WITH EXCITED-STATE TRANSFORMATIONS (ELECTRON, CHARGE, AND PROTON TRANSFERS) DYES WITH SEGMENTAL MOBILITY: MOLECULAR ROTORS ELECTROCHROMISM AND SOLVATOCHROMISM IN FLUORESCENCE RESPONSE OF ORGANIC DYES: A NANOSCOPIC VIEW ELECTRIC FIELD SENSITIVE DYES PART IV FLUOROPHORES OF VISIBLE FLUORESCENT PROTEINS PHOTOPHYSICS AND SPECTROSCOPY OF FLUOROPHORES IN THE GREEN FLUORESCENT PROTEIN FAMILY