BIOLOGÍA CELULAR Y MOLECULAR
CONTENTS 1 APPROACHES AND SPECIES IN THE HISTORY OF VERTEBRATE EMBRYOLOGY 2 MANIPULATING AND IMAGING THE EARLY XENOPUS LAEVIS EMBRYO 3 MANIPULATION OF GENE FUNCTION IN XENOPUS LAEVIS 4 DEVELOPMENTAL GENETICS IN XENOPUS TROPICALIS 5 EMBRYOLOGICAL AND GENETIC MANIPULATION OF CHICK DEVELOPMENT 6 EMBRYOLOGICAL MANIPULATIONS IN ZEBRA?SH 7 PRACTICAL APPROACHES FOR IMPLEMENTING FORWARD GENETIC STRATEGIES IN ZEBRA?SH 8 ESSENTIAL TECHNIQUES FOR INTRODUCING MEDAKA TO A ZEBRA?SH LABORATORY—TOWARDS THE COMBINED USE OF MEDAKA AND ZEBRA?SH FOR FURTHER GENETIC DISSECTION OF THE FUNCTION OF THE VERTEBRATE GENOME 9 EX UTERO CULTURE AND LIVE IMAGING OF MOUSE EMBRYOS 10 DETECTION OF GENE EXPRESSION IN MOUSE EMBRYOS AND TISSUE SECTIONS 11 GENE TARGETING IN THE MOUSE 12 CREATING A “HOPEFUL MONSTER”: MOUSE FORWARD GENETIC SCREENS 13 ASSISTED REPRODUCTIVE TECHNOLOGY IN NONHUMAN PRIMATES 14 EMBRYOLOGICAL METHODS IN ASCIDIANS: THE VILLEFRANCHE-SUR-MER PROTOCOLS 15 CIONA GENETICS 16 ANALYSES OF GENE FUNCTION IN AMPHIOXUS EMBRYOS BY MICROINJECTION OF MRNAS AND MORPHOLINO OLIGONUCLEOTIDES 17 REPTILE EMBRYOLOGY 18 REPRODUCTIVE AND DEVELOPMENTAL MANIPULATION OF THE MARSUPIAL, THE TAMMAR WALLABY MACROPUS EUGENII 19 MUTANT GENERATION IN VERTEBRATE MODEL ORGANISMS BY TILLING 20 INDUCING HIGH RATES OF TARGETED MUTAGENESIS IN ZEBRA?SH USING ZINC FINGER NUCLEASES (ZFNS) 21 DERIVATION OF MOUSE EMBRYONIC STEM CELL LINES FROM BLASTOCYSTS PRODUCED BY FERTILIZATION AND SOMATIC CELL NUCLEAR TRANSFER 22 CLONING MICE AND ES CELLS BY NUCLEAR TRANSFER FROM SOMATIC STEM CELLS AND FULLY DIFFERENTIATED CELLS 23 KEEPING TWO ANIMAL SYSTEMS IN ONE LAB – A FROG PLUS FISH CASE STUDY 24 LABORATORY GUIDELINES FOR ANIMAL CARE