THE PAST AND FUTURE OF PROTEIN PHOSPHATASE RESEARCH
1 Analysis of Protein Phosphatases: Toolbox For Unraveling Cell Signaling Networks 2 A Brief Introduction to the Protein Phosphatase Families 3 Small-Molecule Inhibitors of Ser/Thr Protein Phosphatases: Specificity, Use, and Common Forms of Abuse 4 Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin–Sepharose and Microcystin–Biotin–Sepharose 5 Utilizing Protein Phosphatase Inhibitors to Define PP2A as a Regulator of Ataxia-Telangiectasia Mutated (ATM) 6 An Automated Fluorescence-Based Method for Continuous Assay of PP2A Activity 7 An In Vivo Assay to Quantify Stable Protein Phosphatase 2A (PP2A) Heterotrimeric Species 8 Mutagenesis and Expression of the Scaffolding A? and A? Subunits of PP2A: Assays for Measuring Defects in Binding of Cancer-Related A? and A? Mutants to the Regulatory B and Catalytic C Subunits 9 Isolation and Characterization of PP2A Holoenzymes Containing FLAG-Tagged B Subunits 10 Purification of PP2A Holoenzymes by Sequential Immunoprecipitation with Anti-Peptide Antibodies 1 113 101 85 71 61 47 39 23 9 11 Purification of PP2Ac from Bovine Heart 12 Visualization of Intracellular PP1 Targeting Through Transiently and Stably Expressed Fluorescent Protein Fusions 13 Yeast Two-Hybrid Screens To Identify Drosophila PP1-Binding Proteins 14 Identification of Cellular Protein Phosphatase-1 Regulators 15 Assay for Three-Way Interaction of Protein Phosphatase-1 (Glc7) with Regulatory Subunits Plus Phosphatase Inhibitor-2 16 Phosphorylation of the Protein Phosphatase Type 1 Inhibitor Protein CPI-17 by Protein Kinase C 17 Purification of Smooth Muscle Myosin Phosphatase Using a Thiophosphorylated Myosin Light-Chain-Affinity Resin 18 Proteins Interacting with Saccharomyces cerevisiae Type 1 Protein Phosphatase Catalytic Subunit Identified by Single-Step Affinity Purification and Mass Spectrometry 19 Expression of Protein Histidine Phosphatase in Escherichia coli, Purification, and Determination of Enzyme Activity 20 The Use of RNA Interference to Analyze Protein Phosphatase Function In Mammalian Cells 21 Recognition of a PP2C Interaction Motif in Several Plant Protein Kinases 127 133 155 181 197 209 225 235 247 261 287 22 Use of Yeast Genetic Tools to Define Biological Roles of Novel Protein Phosphatases 23 Targeting of PP2C in Budding Yeast 24 Phosphatase Targets in TOR Signaling 25 Functional Characterization of the Small CTD Phosphatases 26 Genome-Scale Discovery and Characterization of Class-Specific Protein Sequences: An Example Using the Protein Phosphatases of Arabidopsis thaliana 27 Yeast Substrate-Trapping System for Isolating Substrates of Protein Tyrosine Phosphatases 371 347 335 309 299