MOLECULAR BIOLOGY OF THE GENE
CONTENTS 1 PREPARATION AND QUANTI?CATION OF PSEUDOTYPED RETROVIRAL VECTOR 2 PRODUCTION OF RETROVIRAL VECTORS FOR CLINICAL USE 3 METHODS FOR THE PRODUCTION OF HELPER-DEPENDENT ADENOVIRAL VECTORS 4 METHODS FOR THE PRODUCTION OF FIRST GENERATION ADENOVIRAL VECTORS 5 LARGE-SCALE PRODUCTION OF RECOMBINANT ADENO-ASSOCIATED VIRAL VECTORS 6 CONSTRUCTION AND PRODUCTION OF RECOMBINANT HERPES SIMPLEX VIRUS VECTORS 7 PLASMID-BASED GENE TRANSFER IN MOUSE SKELETAL MUSCLE BY ELECTROPORATION 8 CHITOSAN NANOPARTICLE-MEDIATED GENE TRANSFER DONGWON LEE AND SHYAM S MOHAPATRA 9 PEG–PEI COPOLYMERS FOR OLIGONUCLEOTIDE DELIVERY TO CELLS AND TISSUES 10 NON-VIRAL GENE DELIVERY WITH CATIONIC LIPOSOME–DNA COMPLEXES 11 APPLICATIONS OF LENTIVIRAL VECTORS IN NONINVASIVE MOLECULAR IMAGING 12 RETROVIRAL MODI?CATION OF MESENCHYMAL STEM CELLS FOR GENE THERAPY OF HEMOPHILIA A 13 TRANSDUCTION OF MURINE HEMATOPOIETIC STEM CELLS AND IN VIVO SELECTION OF GENE-MODI?ED CELLS 14 ASSESSMENT OF CFTR FUNCTION AFTER GENE TRANSFER IN VITRO AND IN VIVO 15 ONCOLYTIC ADENOVIRUSES FOR CANCER GENE THERAPY 16 DESIGN OF TRANS-SPLICING ADENO-ASSOCIATED VIRAL VECTORS FOR DUCHENNE MUSCULAR DYSTROPHY GENE THERAPY 17 PREVENTION OF TYPE 1 DIABETES IN NOD MICE BY GENETIC ENGINEERING OF HEMATOPOIETIC STEM CELLS 18 LENTIVIRAL VECTOR DELIVERY OF SIRNA AND SHRNA ENCODING GENES INTO CULTURED AND PRIMARY HEMATOPOIETIC CELLS 19 NANOPARTICLE-MEDIATED GENE DELIVERY TO THE LUNG 20 RETROVIRAL-MEDIATED GENE THERAPY FOR THE DIFFERENTIATION OF PRIMARY CELLS INTO A MINERALIZING OSTEOBLASTIC PHENOTYPE 21 IN VIVO SIRNA DELIVERY TO THE MOUSE HYPOTHALAMUS SHOWS A ROLE OF THE CO-CHAPERONE XAP2 IN REGULATING TRH TRANSCRIPTION 22 EF?CIENT RETROVIRAL GENE TRANSFER TO EPIDERMAL STEM CELLS