METHODS IN ENZYMOLOGY - ONCOGENE TECHNIQUES

By J. LASAK

CONTENTS 1 THE IMMORTAL SENESCENCE 2 SENESCENCE PHENOTYPES INDUCED BY RAS IN PRIMARY CELLS 3 CELLULAR MODEL OF P21-INDUCED SENESCENCE 4 DETECTING MARKERS OF THERAPY-INDUCED SENESCENCE IN CANCER CELLS 5 GENOME-WIDE MIRNA SCREENING FOR GENES BYPASSING ONCOGENE-INDUCED SENESCENCE 6 DETECTION OF DYSFUNCTIONAL TELOMERES IN ONCOGENE-INDUCED SENESCENCE 7 RT-QPCR DETECTION OF SENESCENCE-ASSOCIATED CIRCULAR RNAS 8 AUTOPHAGY DETECTION DURING ONCOGENE-INDUCED SENESCENCE USING FLUORESCENCE MICROSCOPY 9 DETECTING THE SENESCENCE-ASSOCIATED SECRETORY PHENOTYPE (SASP) BY HIGH CONTENT MICROSCOPY ANALYSIS 10 SUDAN BLACK B, THE SPECIFIC HISTOCHEMICAL STAIN FOR LIPOFUSCIN: A NOVEL METHOD TO DETECT SENESCENT CELLS 11 USING [U- 13 C 6 ]-GLUCOSE TRACER TO STUDY METABOLIC CHANGES IN ONCOGENE-INDUCED SENESCENCE FIBROBLASTS 12 DETECTION OF THE UBIQUITINOME IN CELLS UNDERGOING ONCOGENE-INDUCED SENESCENCE 13 DETECTION OF REACTIVE OXYGEN SPECIES IN CELLS UNDERGOING ONCOGENE-INDUCED SENESCENCE 14 DETECTION OF SENESCENT CELLS BY EXTRACELLULAR MARKERS USING A FLOW CYTOMETRY-BASED APPROACH 15 METABOLIC CHANGES INVESTIGATED BY PROTON NMR SPECTROSCOPY IN CELLS UNDERGOING ONCOGENE-INDUCED SENESCENCE 16 DETECTION OF NUCLEOTIDE DISBALANCE IN CELLS UNDERGOING ONCOGENE-INDUCED SENESCENCE 17 SENESCENCE-LIKE PHENOTYPES IN HUMAN NEVI 18 DETECTION OF ONCOGENE-INDUCED SENESCENCE IN VIVO 19 DETECTION OF SENESCENCE MARKERS DURING MAMMALIAN EMBRYONIC DEVELOPMENT 20 INDUCTION AND DETECTION OF ONCOGENE-INDUCED CELLULAR SENESCENCE IN DROSOPHILA