CELL BIOLOGY: GENE EXPRESSION (RNA)
PARTI TOOLS FOR CELL-FREE EXPRESSION SYSTEMS 1 Best Practices for DNA Template Preparation Toward Improved Reproducibility in Cell-Free Protein Production 3 2 Simple Extract Preparation Methods for E coli-Based Cell-Free Expression 51 3 Preparation and Screening of Cell-Free Extract from Nongrowing Escherichia coli A19 Cells 65 4 Cell-Free Protein Synthesis Using Pichia pastoris 75 5 AStreptomyces-Based Cell-Free Protein Synthesis System for High-Level Protein Expression 89 6 In Vitro Reconstitution Platforms of Mammalian Cell-Free Expressed Membrane Proteins 105 7 High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems 121 8 Cell-Free Gene Expression from DNA Brushes 135 9 Efficient and Precise Protein Synthesis in a Cell-Free System Using a Set of In Vitro Transcribed tRNAs with Nucleotide Modifications 151 10 Measurement of Transcription, Translation, and Other Enzymatic Processes During Cell-Free Expression Using PERSIA 169 PART II APPLICATIONS OF CELL-FREE EXPRESSION SYSTEMS 11 Cell-Free Noncanonical Redox Cofactor Systems 185 ix 12 Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototyping 199 ¨ 13 Metabolomics Analysis of Cell-Free Expression Systems Using Gas Chromatography-Mass Spectrometry 217 14 Liposome Preparation by 3D-Printed Microcapillary-Based Apparatus 227 15 Microfluidic Production of Porous Polymer Cell-Mimics Capable of Gene Expression 237 16 Cell-Free Membrane Protein Expression into Hybrid Lipid/Polymer Vesicles 257 17 Cell-Free Synthesis Strategies to Probe Co-translational Folding of Proteins Within Lipid Membranes 273 18 Assembly of RNA Nanostructures from Double-Crossover Tiles 293 19 Cell-Free Biosensors and AI Integration 303 20 ROSALIND: Rapid Detection of Chemical Contaminants with In Vitro Transcription Factor-Based Biosensors 325 21 Optical Sensing in Cell-Free Expression 343 22 Cell-Free Paper-Based Analysis of Gut Microbiota and Host Biomarkers 351 23 Detection of Norovirus Using Paper-Based Cell-Free Systems 375 24 A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes 391 25 Implementing Hands-On Molecular and Synthetic Biology Education Using Cell-Free Technology 413