RNAI: DESIGN AND APPLICATION
CONTENTS PART I: DESIGNING OPTIMAL RNAI TOOLS 1 PRINCIPLES OF DICER SUBSTRATE (D-SIRNA) DESIGN AND FUNCTION 2 EXPRESSION, PURI?CATION, AND ANALYSIS OF RECOMBINANT DROSOPHILA DICER-1 AND DICER-2 ENZYMES 1 3 IN VITRO RNA CLEAVAGE ASSAY FOR ARGONAUTE-FAMILY PROTEINS 2 4 IDENTIFYING SIRNA-INDUCED OFF-TARGETS BY MICROARRAY ANALYSIS 4 PART II: APPLICATION OF RNAI IN DIVERSE ORGANISMS 5 HYDRODYNAMIC DELIVERY OF SIRNA IN A MOUSE MODEL OF SEPSIS 6 6 NASAL DELIVERY OF SIRNA 6 7 RNA INTERFERENCE AS A GENETIC TOOL IN TRYPANOSOMES 7 8 LENTIVIRUS-MEDIATED RNA INTERFERENCE IN MAMMALIAN NEURONS 8 9 SILENCING GENES BY RNA INTERFERENCE IN THE PROTOZOAN PARASITE ENTAMOEBA HISTOLYTICA 1 0 10 USE OF RNAI IN C ELEGANS 1 2 11 APPLICATION OF SIRNA AGAINST SARS IN THE RHESUS MACAQUE MODEL 1 3 12 SIRNA AND SHRNA AS ANTICANCER AGENTS IN A CERVICAL CANCER MODEL 1 5 13 IDENTI?CATION AND EXPRESSION ANALYSIS OF SMALL RNAS DURING DEVELOPMENT 1 6 14 SCREENING AND IDENTI?CATION OF VIRUS-ENCODED RNA SILENCING SUPPRESSORS 1 7 15 APPLICATION OF RNA INTERFERENCE IN FUNCTIONAL GENOMICS STUDIES OF A SOCIAL INSECT 1 9 16 TEMPORAL CONTROL OF GENE SILENCING BY IN OVO ELECTROPORATION 2 2 17 ALTERING FLOWER COLOR IN TRANSGENIC PLANTS BY RNAI-MEDIATED ENGINEERING OF FLAVONOID BIOSYNTHETIC PATHWAY 2 3 18 TRANSGENIC RNA INTERFERENCE IN MICE 2 4 I DESIGNING OPTIMAL RNAI TOOLS