BACTERIAL GROWTH AND DIVISION: BIOCHEMISTRY AND REGULATION OF PROKARYOTIC AND EUKARYOTIC DIVISION CYCLES

By M. LAERU

CONTENTS CHROMOSOME CONFORMATION CAPTURE IN BACTERIA AND ARCHAEA MICROCOCCAL NUCLEASE DIGESTION ASSAYS FOR THE ANALYSIS OF CHROMOSOME STRUCTURE IN ARCHAEA DETECTING DNA METHYLATIONS IN THE HYPERTHERMOACIDOPHILIC CRENARCHAEON SULFOLOBUS ACIDOCALDARIUS USING SMRT SEQUENCING PROD: A TOOL FOR PREDICTIVE DESIGN OF TAILORED PROMOTERS IN ESCHERICHIA COLI COMPUTATIONAL STUDY ON THE DYNAMICS OF MYCOBACTERIUM TUBERCULOSIS RNA POLYMERASE ASSEMBLY IN VITRO TRANSCRIPTION ASSAY FOR ARCHAEA BELONGING TO SULFOLOBALES PREDICTION OF DNA-BINDING TRANSCRIPTION FACTORS IN BACTERIA AND ARCHAEA GENOMES IN VIVO SCREENING METHOD FOR THE IDENTI?CATION AND CHARACTERIZATION OF PROKARYOTIC, METABOLITE-RESPONSIVE TRANSCRIPTION FACTORS APPLICATION OF SPECIAL ELECTRON MICROSCOPY TECHNIQUES TO THE STUDY OF DNA – PROTEIN COMPLEXES IN E COLI CELLS HIGH-SPEED ATOMIC FORCE MICROSCOPY VISUALIZATION OF PROTEIN- DNA INTERACTIONS USING DNA ORIGAMI FRAMES SEPARATION AND CHARACTERIZATION OF PROTEIN–DNA COMPLEXES BY EMSA AND IN-GEL FOOTPRINTING CHEMICAL PROTECTION AND PREMODI?CATION-BINDING INTERFERENCE FOR THE IDENTI?CATION OF PHOSPHATE AND BASE-SPECI?C CONTACTS IN PROTEIN–DNA COMPLEXES ANALYSIS OF PROTEIN–DNA INTERACTIONS USING ISOTHERMAL TITRATION CALORIMETRY: SUCCESSES AND FAILURES DETERMINATION OF RNA STRUCTURE WITH IN VITRO SHAPE EXPERIMENTS MICROSCALE THERMOPHORESIS TO STUDY RNA–RNA BINDING AF?NITY TOEPRINT ASSAYS FOR DETECTING RNA STRUCTURE AND PROTEIN–RNA INTERACTIONS