ADVANCES IN GENETICS

By P. JOMII

REQUIREMENT FOR THIS TECHNIQUE IN OTHER WORDS ONLY 7 MER SITES LOCATED ADJACENT TO THE STACKING OLIGONUCLEOTIDES ARE INTERROGATED SINCE THE STACKING HYBRIDIZATION ALLOWS ONLY THEIR DETECTION SINCE ISOLATED 7 MER DUPLEXES NOT STABILIZED BY THE BASE STACKING ARE UNSTABLE UNDER THE HYBRIDIZATION CONDITIONS USED FINALLY IN ORDER TO DECIDE WHETHER ONE AND OTHER METHOD IS ADEQUATE TO DETECT POINT MUTATIONS IT IS IMPORTANT PREVIOUSLY TO KNOW THE MUTATION FREQUENCY EXPECTED FOR ANY NON SELECTED GENE IN NORMAL HUMAN AND IN TUMOR TISSUE WHICH MAKES THE ANALYSIS LESS DIFFICULT MALDONADO RODR GUEZ R ESPINOSA LARA M BARRERA LE N O COLIN TOVAR C GONZ LEZ YEBRA B SALCEDO VARGAS M SANTIAGO HERN NDEZ JC M NDEZ TENORIO A BEATTIE KL DETECTION OF RET PROTO ONCOGENE CODON 634 MUTATIONS USING DOUBLE TANDEM HYBRIDIZATION MOL BIOTECHNOL 2003 25 113 MALDONADO RODR GUEZ R BEATTIE KL ANALYSIS OF NUCLEIC ACIDS BY TANDEM HYBRIDIZATION ON OLIGONUCLEOTIDE MICROARRAYS METHODS MOL BIOL 2001 170 157 171 BEATTIE KL BEATTIE WG MENG L TURNER S VARMA S BISHOP C DAO DD HYBRIDIZATION OF DNA TARGETS TO GLASS TETHERED OLIGONUCLEOTIDE PROBES MOL ANG LICA RANGEL L PEZ ROGELIO MALDONADO RODR GUEZ MAURICIO SALCEDO VARGAS JUANA MERCEDES ESPINOSA LARA ALFONSO M NDEZ TENORIOAND KENNETH L BEATTIE LOW DENSITY DNA MICROARRAY FOR DETECTION OF MOST FREQUENT TP53 MISSENSE POINT MUTATIONS BMC BIOTECHNOLOGY 2005 5