MICROMETHODS IN MOLECULAR BIOLOGY
1 Overview of Advances in Microfluidics and Microfabrication 1 PART I MICROFABRICATION METHODS 2 Introduction to Microfabrication Techniques 5 3 Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds 17 4 Fabrication of Microelectrodes Using the Lift-Off Technique 23 5 Laser Ablation as a Fabrication Technique for Microfluidic Devices 27 6 Reversible, Room Temperature Bonding of Glass Devices for Microfluidics 39 PART II BIOLOGICAL APPLICATIONS 7 Solid-Phase Extraction in Packed Beds on Glass Microdevices 47 8 Fabrication of Micromachined Magnetic Particle Separators for Bioseparation in Microfluidic Systems 65 9 Hydrogel-Immobilized Antibodies for Microfluidic Immunoassays: Hydrogel Immunoassay 83 10 Polymerase Chain Reaction in Miniaturized Systems: Big Progress in Little Devices 97 11 Polymerase Chain Reaction on Microchips 131 12 A Sensitive Sandwich DNA Array Using Fluorescent Nanoparticle Probes 141 13 Microfluidic Ethanol Biobatteries on a Microchip 157 14 Microfluidic Chambers for Cell Migration and Neuroscience Research 167 15 Microtextured Polydimethylsiloxane Substrates for Culturing Mesenchymal Stem Cells 179 16 Contraction Study of a Single Cardiac Muscle Cell in a Microfluidic Chip 199 17 Microscale Integrated Sperm Sorter 227