HUMAN EMBRYONIC STEM CELLS: THE PRACTICAL HANDBOOK

By U. FILAR

CONTENTS SECTION 1: OBTAINING AND CULTURING HUMAN EMBRYONIC STEM CELLS ORGANIZATION AND GOOD ASEPTIC TECHNIQUE IN THE HUMAN EMBRYONIC STEM CELL LABORATORY SOURCING ESTABLISHED HUMAN EMBRYONIC STEM CELL LINES CULTURE OF HUMAN EMBRYOS FOR STEM CELL DERIVATION DERIVATION OF HUMAN EMBRYONIC STEM CELL LINES STANDARD CULTURE OF HUMAN EMBRYONIC STEM CELLS CHEMICALLY-DE?NED CULTURE OF HUMAN EMBRYONIC STEM CELLS SECTION 2: CHARACTERIZATION OF HUMAN EMBRYONIC STEM CELLS PHENOTYPIC ANALYSIS OF HUMAN EMBRYONIC STEM CELLS GENETIC AND EPIGENETIC ANALYSIS OF HUMAN EMBRYONIC STEM CELLS SECTION 3: MANIPULATION OF HUMAN EMBRYONIC STEM CELLS IN VIVO DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS IN VITRO DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO EXTRAEMBRYONIC CELL TYPES DIRECTED DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO ENDODERM: (A) EARLY ENDODERM CELLS (B) HEPATIC CELLS (C) PANCREATIC CELLS DIRECTED DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO MESODERM: (A) CARDIOMYOCYTES (B) ENDOTHELIAL CELLS (C) OSTEOGENIC CELLS (D) HEMATOPOIETIC IN VIVO REPOPULATING CELLS (E) LYMPHOCYTES (F) MYELOID CELLS DIRECTED DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO ECTODERM: (A) FOREBRAIN NEURONS (B) DOPAMINERGIC NEURONS (C) SPINAL MOTOR NEURONS (A) GENE TARGETING IN HUMAN EMBRYONIC STEM CELLS: KNOCK OUT AND KNOCK IN BY HOMOLOGOUS RECOMBINATION (B) RNA INTERFERENCE IN HUMAN EMBRYONIC STEM CELLS (C) GENERATION OF HUMAN GENE REPORTERS USING BACTERIAL ARTI?CIAL CHROMOSOME RECOMBINEERING AFTERWORD