ADVANCED MOLECULAR BIOLOGY: A CONCISE REFERENCE
CONTENTS PART I: REACTIVE OXYGEN AND NITROGEN TECHNIQUES 1 NOVEL DESIGNED PROBES FOR THE CHARACTERIZATION OF OXIDATIVE STRESS IN BIOLOGICAL FLUIDS, CELLS, AND TISSUES 2 A RAPID AND SELECTIVE MASS SPECTROMETRIC METHOD FOR THE IDENTIFICATION OF NITRATED PROTEINS 3 AN EASY AND RELIABLE AUTOMATED METHOD TO ESTIMATE OXIDATIVE STRESS IN THE CLINICAL SETTING 4 CORRELATIVE TRANSMISSION MICROSCOPY: CYTOCHEMICAL LOCALIZATION AND IMMUNOCYTOCHEMICAL LOCALIZATION IN STUDIES OF OXIDATIVE AND NITROSATIVE STRESS 5 DETECTION OF SPECIFICALLY OXIDIZED APOLIPOPROTEINS IN OXIDIZED HDL 6 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY/ELECTRON SPIN RESONANCE/MASS SPECTROMETRY ANALYSES OF LIPID-DERIVED RADICALS 7 EPR SPIN-TRAPPING AND NANO LC MS/MS TECHNIQUES FOR DEPMPO/ OOH AND IMMUNOSPIN-TRAPPING WITH ANTI-DMPO ANTIBODY IN MITOCHONDRIAL ELECTRON TRANSFER SYSTEM 8 DETERMINATION OF HIGH MITOCHONDRIAL MEMBRANE POTENTIAL IN SPERMATOZOA LOADED WITH THE MITOCHONDRIAL PROBE 5, 5,6,6-TETRACHLORO-1,1,3,3- TETRAETHYLBENZIMIDAZOLYL-CARBOCYANINE IODIDE (JC-1) AND FOR USING FLUORESCENCE-ACTIVATED FLOW CYTOMETRY 9 2,2,6,6-TETRAMETHYLPIPERIDIN-1-OXYL PROBES FOR EVALUATING OXIDATIVE STRESS ON THE CELL MEMBRANE AND MITOCHONDRIA 10 ZYMOGRAPHICAL TECHNIQUES FOR DETECTION OF MATRIX METALLOPROTEINASES 16 ANTIOXIDANT QSAR MODELING AS EXEMPLIFIED ON POLYPHENOLS 17 IMMUNOHISTOCHEMICAL STAINING OF CYCLOOXYGENASES WITH MONOCLONAL ANTIBODIES 18 EXAMINING THE ENDOGENOUS ANTIOXIDANT RESPONSE THROUGH IMMUNOFLUORESCENT ANALYSIS OF NRF2 IN TISSUE 19 DETERMINATION OF OXIDIZED AND REDUCED COQ10 AND COQ9 IN HUMAN PLASMA/SERUM USING HPLC-ECD 20 PARAOXONASES (PON1, PON2, PON3) ANALYSIS IN VITRO AND IN VIVO IN RELATION TO CARDIOVASCULAR DISEASES 21 PREPARATION, CHARACTERIZATION, AND USE OF ANTIOXIDANT-LIPOSOMES 22 ANTIOXIDANT ACTIVITY OF BIOTRANSFORMED SEX HORMONES FACILITATED BY BACILLUS STEAROTHERMOPHILUS 23 BIOLISTIC MEDIATED PRODUCTION OF TRANSGENIC OIL PALM 24 PREPARATION OF A MULTI-ANTIOXIDANT FORMULATION PART III: GENE EXPRESSION 25 A FUNCTIONAL GENOMICS APPROACH TO IDENTIFY AND CHARACTERIZE OXIDATION RESISTANCE GENES 26 GENOME-WIDE OVEREXPRESSION SCREEN FOR ACTIVATORS OF ANTIOXIDANT GENE TRANSCRIPTION 27 METHOD FOR CONDUCTING MICROARRAY STUDY OF OXIDATIVE STRESS INDUCED GENE EXPRESSION 28 A SYSTEMS APPROACH DEMONSTRATING SPHINGOLIPID-DEPENDENT TRANSCRIPTION IN STRESS RESPONSES 29 BIOLUMINESCENCE: IMAGING MODALITY FOR IN VITRO AND IN VIVO GENE EXPRESSION PART IV: STEM CELLS 30 REACTIVE OXYGEN SPECIES AND UPREGULATION OF NADPH OXIDASES IN MECHANOTRANSDUCTION OF EMBRYONIC STEM CELLS PART V: BIOSTATISTICS 31 POOLING DATA WHEN ANALYZING BIOMARKERS SUBJECT TO A LIMIT OF DETECTION