GENETIC COMPLEMENTATION PROTOCOLS

By D. LAVAM

1 GENETIC COMPLEMENTATION PROTOCOLS 2 USE OF POL I-DEFICIENT E COLI FOR FUNCTIONAL COMPLEMENTATION OF DNA POLYMERASE 3 SELECTION OF NOVEL EUKARYOTIC DNA POLYMERASES BY MUTAGENESIS AND GENETIC COMPLEMENTATION OF YEAST 4 AUTOGENE SELECTIONS 5 SELECTION FOR SOLUBLE PROTEINS VIA FUSION WITH CHLORAMPHENICOL ACETYLTRANSFERASE 6 PROSIDE 7 MINIMIZATION OF PROTEINS BY RANDOM FRAGMENTATION AND SELECTION 8 EVALUATING A SCREEN AND ANALYSIS OF MUTANT LIBRARIES 9 SCREENING MUTANT LIBRARIES IN SACCHAROMYCES CEREVISIAE 10 SOLID-PHASE SCREENING USING DIGITAL IMAGE ANALYSIS 11 SCREENING FOR THERMOSTABILITY 12 HIGH-THROUGHPUT SCREENING OF MUTANT ?-AMYLASE LIBRARIES FOR INCREASED ACTIVITY AT 129°C 13 HIGH-THROUGHPUT CARBON MONOXIDE BINDING ASSAY FOR CYTOCHROMES P450 14 HIGH-THROUGHPUT SCREEN FOR AROMATIC HYDROXYLATION CONTENTS 15 COLORIMETRIC SCREEN FOR ALIPHATIC HYDROXYLATION BY CYTOCHROME P450 USING P-NITROPHENYL-SUBSTITUTED ALKANES 16 HIGH-THROUGHPUT SCREENS BASED ON NAD(P)H DEPLETION 17 HIGH-THROUGHPUT TETRAMETHYLBENZIDINE (TMB) SCREEN FOR PEROXIDASES 18 SCREEN FOR OXIDASES BY DETECTION OF HYDROGEN PEROXIDE WITH HORSERADISH PEROXIDASE 19 COLORIMETRIC DEHYDROGENASE SCREEN BASED ON NAD(P)H GENERATION 20 COLORIMETRIC ASSAYS FOR SCREENING LACCASES 21 PH SENSING AGAR PLATE ASSAYS FOR ESTEROLYTIC ENZYME ACTIVITY 22 A PH-INDICATOR-BASED SCREEN FOR HYDROLYTIC HALOALKANE DEHALOGENASE 23 DETECTION OF AROMATIC ?-HYDROXYKETONES WITH TETRAZOLIUM SALTS 24 SELECTION OF HEAT-STABLE CLOSTRIDIUM CELLULOVORANS CELLULASES AFTER IN VITRO RECOMBINATION 25 SCREENING AND SELECTION STRATEGIES FOR DISULFIDE ISOMERASE ACTIVITY 26 AN OVERVIEW OF HIGH-THROUGHPUT SCREENING SYSTEMS FOR ENANTIOSELECTIVE ENZYMATIC TRANSFORMATIONS 27 SELECT PROTOCOLS OF HIGH-THROUGHPUT EE-SCREENING SYSTEMS FOR ASSAYING ENANTIOSELECTIVE ENZYMES 28 DIRECTED EVOLUTION OF THE SUBSTRATE SPECIFICITIES OF A SITE-SPECIFIC RECOMBINASE AND AN AMINOACYL-TRNA SYNTHETASE USING FLUORESCENCE-ACTIVATED CELL SORTING (FACS) 29 CALMODULIN-TAGGED PHAGE AND TWO-FILTER SANDWICH ASSAYS FOR THE IDENTIFICATION OF ENZYMATIC ACTIVITIES 30 HIGH-THROUGHPUT FACS METHOD FOR DIRECTED EVOLUTION OF SUBSTRATE SPECIFICITY 31 IMPROVING PROTEIN FOLDING EFFICIENCY BY DIRECTED EVOLUTION USING THE GFP FOLDING REPORTER