FUNGAL LIPID BIOCHEMISTRY
CONTENTS 1 METHOD FOR ASSAYING THE LIPID KINASE PHOSPHATIDYLINOSITOL-5- PHOSPHATE 4-KINASE ? IN QUANTITATIVE HIGH-THROUGHPUT SCREENING (QHTS) BIOLUMINESCENT FORMAT 2 ASSAYING CERAMIDE SYNTHASE ACTIVITY IN VITRO AND IN LIVING CELLS USING LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY 3 FLUORESCENT ASSAYS FOR CERAMIDE SYNTHASE ACTIVITY 4 IDENTIFICATION OF THE INTERACTOME OF A PALMITOYLATED MEMBRANE PROTEIN, PHOSPHATIDYLINOSITOL 4-KINASE TYPE II ALPHA 5 MEASUREMENT OF LONG-CHAIN FATTY ACYL-COA SYNTHETASE ACTIVITY 6 QUALITATIVE AND QUANTITATIVE IN VITRO ANALYSIS OF PHOSPHATIDYLINOSITOL PHOSPHATASE SUBSTRATE SPECIFICITY 7 LUCIFERASE REPORTER ASSAYS TO ASSESS LIVER X RECEPTOR TRANSCRIPTIONAL ACTIVITY 8 METABOLICALLY BIOTINYLATED REPORTERS FOR ELECTRON MICROSCOPIC IMAGING OF CYTOPLASMIC MEMBRANE MICRODOMAINS 9 FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING ANALYSIS OF THE DIFFUSIONAL MOBILITY OF PLASMA MEMBRANE PROTEINS: HER3 MOBILITY IN BREAST CANCER CELL MEMBRANES 10 ISOLATION AND ANALYSIS OF DETERGENT-RESISTANT MEMBRANE FRACTIONS 11 DETECTION OF ISOLATED MITOCHONDRIA-ASSOCIATED ER MEMBRANES USING THE SIGMA-1 RECEPTOR 12 USING SURFACE PLASMON RESONANCE TO QUANTITATIVELY ASSESS LIPID–PROTEIN INTERACTIONS 13 ANALYZING PROTEIN–PHOSPHOINOSITIDE INTERACTIONS WITH LIPOSOME FLOTATION ASSAYS 14 HIGH-THROUGHPUT FLUOROMETRIC ASSAY FOR MEMBRANE–PROTEIN INTERACTION 15 GUIDELINES FOR THE USE OF PROTEIN DOMAINS IN ACIDIC PHOSPHOLIPID IMAGING 16 ANALYSIS OF SPHINGOLIPID SYNTHESIS AND TRANSPORT BY METABOLIC LABELING OF CULTURED CELLS WITH [3H]SERINE 17 DETERMINATION AND CHARACTERIZATION OF TETRASPANIN- ASSOCIATED PHOSPHOINOSITIDE-4 KINASES IN PRIMARY AND NEOPLASTIC LIVER CELLS 18 ANALYSIS OF THE PHOSPHOINOSITIDE COMPOSITION OF SUBCELLULAR MEMBRANE FRACTIONS 19 SINGLE-MOLECULE IMAGING OF SIGNAL TRANSDUCTION VIA GPI- ANCHORED RECEPTORS 20 MEASURING PHOSPHATIDYLINOSITOL GENERATION ON BIOLOGICAL MEMBRANES 21 ASSAY FOR CDP-DIACYLGLYCEROL GENERATION BY CDS IN MEMBRANE FRACTIONS