BIOCHEMISTRY AND MOLECULAR BIOLOGY OF FISHES: ANALYTICAL TECHNIQUES

By S. FILAE

FIXATION/ PERMEABILIZATION THERE ARE SEVERAL AGENTS THAT CAN BE EMPLOYED DURING ?XATION/ PERMEABILIZATION THE CHOICE FOR THE AGENTS TO BE USED IN THIS STEP IS MOSTLY DEPENDENT ON THREE FACTORS: THE TYPE OF MICROORGANISM (MAINLY THE CELL ENVELOPE), THE TYPE OF NUCLEIC ACID PROBE USED, AND THE TARGET CELLS’ SPATIAL LOCATION (I E , WHEREAS CELLS ARE IN SLIDES, ?LTER TABLE VISUALIZATION/ DETECTION SAMPLE AUTO?UORESCENCE TYPE OF SAMPLE AND MOUNTING MEDIA EQUIPMENT FLUORESCENT ?LTERS AND LIGHT SOURCE FLUORESCENCE DETECTION LIMIT EQUIPMENT SETTINGS; ?UOROCHROME PROPERTIES FILTER WAVELENGTH BAND PASS/LONG PASS, ?T TO THE ?UOROCHROMES EXCITATION AND EMISSION SPECTRA, AND CROSS TALK TYPE OF FLUOROCHROME CY3, FITC, ALEXA FLUOR FLUOROCHROME QUENCHING USER EXPERTISE, TYPE OF ?UOROCHROME, AND LIGHT SOURCE BIOCHEMISTRY AND MOLECULAR BIOLOGY OF FISHES, 3 ANALYTICAL TECHNIQUES