CDNA FROM SINGLE CELLS AND SUBCELLULAR REGIONS

By B. DIINU

CONTENTS IMMUNOCHEMISTRY ANALYSIS USING CHROMOGENIC SUBSTRATES ON TISSUE SECTIONS INDIRECT IMMUNO?UORESCENCE OF TISSUE SECTIONS FLOW CYTOMETRY FOR BEGINNERS: HINTS AND TIPS FOR APPROACHING THE VERY FIRST SINGLE-CELL TECHNIQUE HIGH-DIMENSIONAL IMMUNOPHENOTYPING WITH 37-COLOR PANEL USING FULL-SPECTRUM CYTOMETRY DETECTION OF CYTOKINE-SECRETING CELLS BY ENZYME-LINKED IMMUNOSPOT (ELISPOT) DETECTION AND ENUMERATION OF CYTOKINE-SECRETING CELLS BY FLUOROSPOT SINGLE-CELL PROTEIN PRO?LING BY MICRODROPLET BARCODING AND NEXT-GENERATION SEQUENCING SINGLE CELL PROTEOMICS USING MULTIPLEXED ISOBARIC LABELING FOR MASS SPECTROMETRIC ANALYSIS CUSTOMIZING MAXPAR DIRECT IMMUNE PRO?LING ASSAY WITH ADDITIONAL SURFACE MARKER AND INTRACELLULAR CYTOKINE STAINING WORK?OWS FOR EXPANDED MASS CYTOMETRY PANELS HIGH-DIMENSIONAL TISSUE PRO?LING BY MULTIPLEXED ION BEAM IMAGING ULTRA-SENSITIVE QUANTI?CATION OF PROTEIN AND MRNA IN SINGLE MAMMALIAN CELLS WITH DIGITAL PLA HIGH-THROUGHPUT MULTIMODAL SINGLE-CELL TARGETED DNA AND SURFACE PROTEIN ANALYSIS USING THE MISSION BIO TAPESTRI PLATFORM SIMULTANEOUS ANALYSIS OF SINGLE-CELL TRANSCRIPTOMES AND CELL SURFACE PROTEIN EXPRESSION OF HUMAN HEMATOPOIETIC STEM CELLS AND PROGENITORS USING THE 10X GENOMICS PLATFORM GENERATION OF CENTERED LOG-RATIO NORMALIZED ANTIBODY-DERIVED TAG COUNTS FROM LARGE SINGLE-CELL SEQUENCING DATASETS SIMULTANEOUS QUANTI?CATION OF SINGLE-CELL PROTEOMES AND TRANSCRIPTOMES IN INTEGRATED FLUIDIC CIRCUITS COMBINED MEASUREMENT OF RNA AND PROTEIN EXPRESSION ON A SINGLE-CELL LEVEL SIMULTANEOUS DNA, RNA, AND PROTEIN ANALYSIS FROM SINGLE CELLS USING A HIGH-THROUGHPUT MICRO?UIDIC WORK?OW FOR RESOLUTION OF GENOTYPE-TO-PHENOTYPE MODALITIES