ISOLATED HEPATOCYTES: PREPARATION, PROPERTIES AND APPLICATIONS
PART I IN VITRO MODELS OF THE HEPATOCYTE LIFE CYCLE 1 ISOLATION AND CULTURE OF MOUSE HEPATOCYTES: GENDER- SPECIFIC GENE EXPRESSION RESPONSES TO CHEMICAL TREATMENTS 2 CRYOPRESERVATION OF HEPATOCYTES 3 CULTURE CONDITIONS PROMOTING HEPATOCYTE PROLIFERATION AND CELL CYCLE SYNCHRONIZATION 4 IMMORTALIZED HUMAN HEPATIC CELL LINES FOR IN VITRO TESTING AND RESEARCH PURPOSES 5 CULTURE AND FUNCTIONAL CHARACTERIZATION OF HUMAN HEPATOMA HEPG2 CELLS 6 ESTABLISHMENT AND CHARACTERIZATION OF AN IN VITRO MODEL OF FAS-MEDIATED HEPATOCYTE CELL DEATH 7 SERUM-FREE DIRECTED DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO HEPATOCYTES 8 HUMAN SKIN-DERIVED PRECURSOR CELLS: ISOLATION, EXPANSION, AND HEPATIC DIFFERENTIATION 9 GENERATION OF HEPATOCYTES FROM PLURIPOTENT STEM CELLS FOR DRUG SCREENING AND DEVELOPMENTAL MODELING 10 DIFFERENTIATION-PROMOTING MEDIUM ADDITIVES FOR HEPATOCYTE CULTIVATION AND CRYOPRESERVATION 11 COCULTURE AND LONG-TERM MAINTENANCE OF HEPATOCYTES CONTENTS 12 PRIMARY HEPATOCYTES IN SANDWICH CULTURE 13 ESTABLISHING LIVER BIOREACTORS FOR IN VITRO RESEARCH 14 EPIGENETIC MODIFICATIONS AS ANTIDEDIFFERENTIATION STRATEGY FOR PRIMARY HEPATOCYTES IN CULTURE 15 TRANSFECTION OF PRIMARY HEPATOCYTES WITH LIVER-ENRICHED TRANSCRIPTION FACTORS USING ADENOVIRAL VECTORS PART II IN VITRO METHODS TO PROBE HEPATOCYTE FUNCTIONALITY AND TOXICITY 16 TRANSCRIPTOMICS OF HEPATOCYTES TREATED WITH TOXICANTS FOR INVESTIGATING MOLECULAR MECHANISMS UNDERLYING HEPATOTOXICITY 17 GLOBAL MICRORNA ANALYSIS IN PRIMARY HEPATOCYTE CULTURES 18 MASS SPECTROMETRY-BASED PROTEOMICS FOR RELATIVE PROTEIN QUANTIFICATION AND BIOMARKER IDENTIFICATION IN PRIMARY HUMAN HEPATOCYTES 19 TARGETED METABOLOMICS FOR HOMOCYSTEINE-RELATED METABOLITES IN PRIMARY HEPATOCYTES 20 MEASUREMENT OF CYTOCHROME P450 ENZYME INDUCTION AND INHIBITION IN HUMAN HEPATOMA CELLS 21 ANALYSIS OF SINUSOIDAL DRUG UPTAKE TRANSPORTER ACTIVITIES IN PRIMARY HUMAN HEPATOCYTES 22 MEASUREMENT OF ALBUMIN SECRETION AS FUNCTIONALITY TEST IN PRIMARY HEPATOCYTE CULTURES 23 MEASUREMENT OF BLOOD COAGULATION FACTOR SYNTHESIS IN CULTURES OF HUMAN HEPATOCYTES 24 FUNCTIONALITY TESTING OF PRIMARY HEPATOCYTES IN CULTURE BY MEASURING UREA SYNTHESIS 25 ASSAY OF BILE ACID CONJUGATION AND EXCRETION IN HUMAN HEPATOCYTES 26 GENERAL CYTOTOXICITY ASSESSMENT BY MEANS OF THE MTT ASSAY 27 MEASUREMENT OF APOPTOTIC AND NECROTIC CELL DEATH IN PRIMARY HEPATOCYTE CULTURES 28 CRITICAL FACTORS IN THE ASSESSMENT OF CHOLESTATIC LIVER INJURY IN VITRO 29 IN VITRO CELL CULTURE MODELS OF HEPATIC STEATOSIS 30 ASSESSMENT OF LIVER FIBROTIC INSULTS IN VITRO